Savasta M, Dubois A, Benavides J, Scatton B
Neurosci Lett. 1986 Dec 23;72(3):265-71. doi: 10.1016/0304-3940(86)90524-0.
The precise neuronal localization of D1 receptors in the substantia nigra has been studied autoradiographically in the rat by measuring the alterations of [3H]SCH 23390 binding site densities in this brain area after 6-hydroxydopamine (6-OHDA) induced destruction of nigrostriatal dopaminergic neurons and after ibotenate-induced lesion of striatal afferents. 6-OHDA-induced nigral lesion provoked a total loss of [3H]SCH 23390 binding sites in the pars compacta and pars lateralis (but not in the pars reticulata) of the substantia nigra. In contrast, ibotenate-induced striatal lesion caused a large diminution of the [3H]ligand binding site density in the pars reticulata but not in the pars compacta and pars lateralis of the substantia nigra. These results suggest that D1 receptors in the pars compacta or pars lateralis of the substantia nigra are located on the dopaminergic perikarya whereas those D1 receptors present in the pars reticulata of the substantia nigra lie on the terminals of nigral afferents of striatal origin.
通过测量6-羟基多巴胺（6-OHDA）诱导黑质纹状体多巴胺能神经元破坏后以及鹅膏蕈氨酸诱导纹状体传入纤维损伤后，大鼠脑区中[3H]SCH 23390结合位点密度的变化，利用放射自显影技术研究了黑质中D1受体的精确神经元定位。6-OHDA诱导的黑质损伤导致黑质致密部和外侧部（但不是网状部）的[3H]SCH 23390结合位点完全丧失。相反，鹅膏蕈氨酸诱导的纹状体损伤导致黑质网状部的[3H]配体结合位点密度大幅降低，但黑质致密部和外侧部没有。这些结果表明，黑质致密部或外侧部的D1受体位于多巴胺能神经元胞体上，而黑质网状部的那些D1受体则位于纹状体起源的黑质传入纤维终末上。
