Borzenok S A, Afanas'eva D S, Gushchina M B, Ostrovskii D S, Domogatsky S P, Osidak E O
S. N. Fyodorov Eye Microsurgery Federal State Institution, Ministry of Health of the Russian Federation, Moscow, Russia.
A. I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2018 Mar;164(4):543-549. doi: 10.1007/s10517-018-4029-6. Epub 2018 Mar 4.
The interplay of multipotent stromal cells derived from the orbital fat pads and cells of the lipoaspirate from the subcutaneous adipose tissue was studied using in vitro co-transplantation model in an organ culture in a collagen gel. Microscopy findings and intensity of apoptosis and cell proliferation in cultures of lipoaspirate with and without multipotent stromal cells showed that the cells maintained their viability, proliferation capacity, and cytokine secretion activity. Higher proliferatitive activity of cells in cocultures promotes renewal of fat transplant cells and can help to maintain its stable volume in delayed terms after transplantation.
利用胶原凝胶器官培养中的体外共移植模型，研究了源自眼眶脂肪垫的多能基质细胞与皮下脂肪组织脂肪抽吸物中的细胞之间的相互作用。对有和没有多能基质细胞的脂肪抽吸物培养物进行显微镜检查、凋亡强度和细胞增殖研究，结果表明这些细胞保持了它们的活力、增殖能力和细胞因子分泌活性。共培养中细胞较高的增殖活性促进了脂肪移植细胞的更新，并有助于在移植后的延迟期维持其稳定体积。
