体外多核巨噬细胞中白细胞介素-1α、碱性成纤维细胞生长因子、转化生长因子-β1、干扰素γ、基质金属蛋白酶-1和组织蛋白酶D表达的分析
Analysis of IL-1α, bFGF, TGF-β1, IFNγ, MMP-1, and CatD Expression in Multinuclea Macrophages In Vitro.
作者信息
Il'in D A, Arkhipov S A, Shkurupy V A
机构信息
Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia.
Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
出版信息
Bull Exp Biol Med. 2018 Mar;164(4):456-458. doi: 10.1007/s10517-018-4011-3. Epub 2018 Mar 5.
The incidence of mono- and multinuclear cells and their expression of pro- and antifibrotic factors were studied in cultured peritoneal macrophages from intact and BCG-infected mice. Generally, the expression of factors increased with an increase in the number of nuclei per cell. However, the expression was higher in macrophages from BCG infected mice, except the cells with 3 and more nuclei, extremely rarely expressing IL-1α in cultures from intact and BCG-infected animals. The number of macrophages with 3 and more nuclei, expressing CatD, was comparable with the number of mono- and binuclear macrophages. Presumably, this was determined by various mechanisms of formation of multinuclear (3-5 and more nuclei) macrophages, for example, by amitosis.
在来自未感染和卡介苗感染小鼠的培养腹膜巨噬细胞中，研究了单核和多核细胞的发生率及其促纤维化和抗纤维化因子的表达。一般来说，因子的表达随着每个细胞中细胞核数量的增加而增加。然而，除了具有3个及更多细胞核的细胞外，卡介苗感染小鼠的巨噬细胞中因子表达更高，在未感染和卡介苗感染动物的培养物中，具有3个及更多细胞核的细胞极少表达IL-1α。表达组织蛋白酶D的具有3个及更多细胞核的巨噬细胞数量与单核和双核巨噬细胞数量相当。据推测，这是由多核（3 - 5个及更多细胞核）巨噬细胞形成的各种机制决定的，例如通过无丝分裂。
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