Kuhl P, Olek K, Wardenbach P, Grzeschik K H
Hum Genet. 1979;53(1):47-9. doi: 10.1007/BF00289450.
An acrylamide gel electrophoretic procedure is described which allows the separation of human quinoid-dihydropteridine reductase (QDPR), EC 1.6.5.1) from the homologous enzyme expressed in established rodent cell lines. The human enzyme marker segregates exclusively with chromosome 4 in a series of well characterized man-mouse somatic cell hybrid clones from our clone bank. This observation supports the assignment of a structural gene for QDPR to human chromosome 4.
本文描述了一种丙烯酰胺凝胶电泳方法,该方法可将人类醌二氢蝶啶还原酶(QDPR,EC 1.6.5.1)与在已建立的啮齿动物细胞系中表达的同源酶分离。在我们克隆库中一系列特征明确的人-鼠体细胞杂交克隆中,人类酶标记仅与4号染色体分离。这一观察结果支持将QDPR的结构基因定位于人类4号染色体。