Kaviani Rosa, Londono Irene, Parent Stefan, Moldovan Florina, Villemure Isabelle
Mechanical Engineering Department, Institute of Biomedical Engineering, École Polytechnique of Montreal, Montreal, Quebec, Canada.
J Musculoskelet Neuronal Interact. 2018 Mar 1;18(1):81-91.
The objective of this study was to investigate the effects of mechanical modulation parameters on structural proteins biocomposition and mechanical properties of the growth plate. Establishing these parameters is a crucial step in the development of fusionless treatment of scoliosis. In this study, ulna explants from 4-weeks-old (pubertal) swines were used. The biocomposition was characterized using biochemical content evaluation and immunohistochemistry. Mechanical properties were characterized by fitting the data of the stress relaxation curves using a fibril reinforced biphasic model. For the mechanical loading, one static modulation condition and three different dynamic modulation conditions, with similar average stress but different amplitude and frequency values, were performed using a bioreactor. Results showed that static loading triggers a decrease in proteoglycan content and type X collagen in specific zones of the growth plate. These changes can be associated with the observed decrement of permeability in the static group. None of the three conditions evaluated for dynamic modulation affected the growth plate biocomposition and biomechanical responses. Results of this study provides an improved understanding of growth plate responses to mechanical environment, which will be useful in finding the optimal and non-damaging parameters for fusionless treatments based on the mechanical modulation of bone growth.
本研究的目的是调查机械调节参数对生长板结构蛋白生物组成和力学性能的影响。确定这些参数是脊柱侧弯非融合治疗发展中的关键一步。在本研究中，使用了4周龄（青春期）猪的尺骨外植体。通过生化含量评估和免疫组织化学对生物组成进行表征。使用纤维增强双相模型拟合应力松弛曲线数据来表征力学性能。对于机械加载，使用生物反应器进行一种静态调节条件和三种不同的动态调节条件，它们具有相似的平均应力但振幅和频率值不同。结果表明，静态加载会导致生长板特定区域蛋白聚糖含量和X型胶原蛋白减少。这些变化可能与静态组中观察到的通透性降低有关。评估的三种动态调节条件均未影响生长板生物组成和生物力学反应。本研究结果有助于更好地理解生长板对机械环境的反应，这将有助于找到基于骨生长机械调节的非融合治疗的最佳且无损伤的参数。
