N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia.
Research Institute of Pulmonology, Moscow, Russia.
BMC Genomics. 2018 Feb 9;19(Suppl 3):74. doi: 10.1186/s12864-018-4472-9.
Microbes infecting cystic fibrosis patients' respiratory tract are important in determining patients' functional status. Representatives of Burkholderiales order are the most dangerous. The goal of our investigation was to reveal the diversity of Burkholderiales, define of their proportion in the microbiome of various parts of respiratory tract and determine the pathogenicity of the main representatives.
In more than 500 cystic fibrosis patients, representing all Federal Regions of Russia, 34.0% were infected by Burkholderia cepacia complex (Bcc), 21.0% by Achromobacter spp. and 12.0% by Lautropia mirabilis. B. cenocepacia was the most numerous species among the Bcc (93.0%), and A. ruhlandii was the most numerous among Achromobacter spp. (58.0%). The most abundant genotype in Bcc was sequence type (ST) 709, and in Achromobacter spp. it was ST36. These STs constitute Russian epidemic strains. Whole genome sequencing of strains A. ruhlandii SCCH3:Ach33-1365 ST36 and B. cenocepacia GIMC4560:Bcn122 ST709 revealed huge resistomes and many virulence factors, which may explain the difficulties in eradicating these strains. An experience of less dangerous B. cenocepcia ST710 elimination was described. Massively parallel sequencing of 16S rDNA amplicons, including V1-V4 hypervariable regions, was used to definite "healthy" microbiome characteristics. Analysis of maxillary sinus lavage of 7 patients revealed infection with Proteobacteria of the same ST as pathogens from sputum, suggesting that the maxillary sinus is a source of infection in cystic fibrosis patients.
Characterization of the Russian epidemic bacterial strains in the sputum and sinuses of cystic fibrosis patients have better defined the importance of Burkholderiales bacteria. This information may aid in the development of effective approaches for treatment of this disease.
感染囊性纤维化患者呼吸道的微生物在确定患者的功能状态方面起着重要作用。伯克霍尔德氏菌目代表的微生物是最危险的。我们的研究目标是揭示伯克霍尔德氏菌目的多样性，确定其在呼吸道不同部位微生物组中的比例，并确定主要代表菌的致病性。
在来自俄罗斯所有联邦区的 500 多名囊性纤维化患者中，34.0%感染了洋葱伯克霍尔德氏复合群（Bcc），21.0%感染了不动杆菌属，12.0%感染了罗威氏菌属。在 Bcc 中，最常见的物种是洋葱伯克霍尔德氏菌（B. cenocepacia）（93.0%），在不动杆菌属中，最常见的物种是鲁氏不动杆菌（A. ruhlandii）（58.0%）。Bcc 中最丰富的基因型是序列型（ST）709，不动杆菌属中最丰富的基因型是 ST36。这些 ST 构成了俄罗斯的流行株。鲁氏不动杆菌属菌株 A. ruhlandii SCCH3:Ach33-1365 ST36 和洋葱伯克霍尔德氏菌属菌株 B. cenocepacia GIMC4560:Bcn122 ST709 的全基因组测序揭示了巨大的耐药组和许多毒力因子，这可能解释了根除这些菌株的困难。描述了一种消除危害较小的洋葱伯克霍尔德氏菌属 ST710 的经验。使用包括 V1-V4 高变区在内的 16S rDNA 扩增子的大规模平行测序来确定“健康”微生物组的特征。对 7 名患者上颌窦灌洗液的分析显示，与痰中病原体相同 ST 的变形菌门感染，表明上颌窦是囊性纤维化患者感染的来源。
对囊性纤维化患者痰和窦中的俄罗斯流行菌株进行特征描述，更好地确定了伯克霍尔德氏菌的重要性。这些信息可能有助于开发治疗这种疾病的有效方法。
