Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, Germany.
Buchmann Institute for Molecular Life Sciences, Goethe-Universität Frankfurt, Frankfurt am Main, Germany.
Angew Chem Int Ed Engl. 2018 May 14;57(20):5699-5702. doi: 10.1002/anie.201713199. Epub 2018 Apr 14.
Photorhabdus luminescens dedicates a significant proportion of its genome to the production of natural products. These products and the structural variation in their derivatives may occur by a number of well-described mechanisms, such as module skipping or precursor promiscuity. Cappable-seq was used to identify transcriptional start sites of many of the gene clusters present in P. luminescens TTO1. We discovered that variations associated with the non-ribosomal peptide synthetase Kol, which is responsible for kolossin A production, possessed a number of internal transcripts that lead to synthesis of the smaller kolossin derivatives kolossin B and C. The data here support a new mechanism of natural product biosynthetic variation whereby mRNA may code for shorter NRPS enzymes in addition to full-length proteins, resulting in the production of smaller peptide derivatives.
发光杆菌 dedicates 其基因组的很大一部分用于天然产物的生产。这些产物及其衍生物的结构变化可能通过多种描述良好的机制发生，例如模块跳过或前体混杂。Cappable-seq 被用于鉴定存在于 P. luminescens TTO1 中的许多基因簇的转录起始位点。我们发现，与负责 kolossin A 产生的非核糖体肽合酶 Kol 相关的变异具有许多内部转录物，这些转录物导致较小的 kolossin 衍生物 kolossin B 和 C 的合成。这里的数据支持一种新的天然产物生物合成变异机制，即 mRNA 除了全长蛋白外，还可能编码较短的 NRPS 酶，从而产生较小的肽衍生物。
