Mennetrier Lucas, Lopez Tatiana, Pruvot Benoist, Yousfi Nadhir, Armant Olivier, Hazhaz Hanae, Lhuissiez Vincent, Garrido Carmen, Chluba Johanna
a UFR SVTE - UFR Sciences de la Vie, de la Terre et de l'Environnement, Université de Bourgogne Franche-Comté , Dijon , France.
b UMR 1231 INSERM LNC , Dijon , France.
Chronobiol Int. 2018 Jul;35(7):1008-1015. doi: 10.1080/07420528.2018.1441855. Epub 2018 Mar 6.
HSF1 is a transcription factor that plays a key role in circadian resetting by temperature. We have used zebrafish embryos to decipher the roles of zHsf1, heat and light on zper2 transcription in vivo. Our results show that heat shock (HS) stimulated zper2 expression in the dark but has no cumulative effect combined with light. After light exposition, zper2 expression was 2.7 fold increased threefold in the hsf1-morphants in comparison to control embryos. Our results show that zHsf1 plays a positive role in HS-driven expression of zper2 in the dark but seems to act as an attenuator in the presence light.
热休克因子1（HSF1）是一种转录因子，在温度介导的昼夜节律重置中起关键作用。我们利用斑马鱼胚胎在体内解析了斑马鱼热休克因子1（zHsf1）、热和光对zper2转录的作用。我们的结果表明，热休克（HS）在黑暗中刺激zper2表达，但与光联合时无累积效应。光照后，与对照胚胎相比，hsf1 - morphants（基因敲降胚胎）中zper2表达增加了2.7倍。我们的结果表明，zHsf1在黑暗中对HS驱动的zper2表达起正向作用，但在有光的情况下似乎起衰减作用。
