São Carlos Institute of Chemistry, University of São Paulo, São Carlos, SP 13560-970, Brazil.
São Carlos Institute of Chemistry, University of São Paulo, São Carlos, SP 13560-970, Brazil.
Bioelectrochemistry. 2018 Aug;122:11-25. doi: 10.1016/j.bioelechem.2018.02.007. Epub 2018 Feb 27.
Integrating in situ biofuel production and energy conversion into a single system ensures the production of more robust networks as well as more renewable technologies. For this purpose, identifying and developing new biocatalysts is crucial. Herein, is reported a bioelectrochemical system consisting of alcohol dehydrogenase (ADH) and Saccharomyces cerevisiae, wherein both function cooperatively for ethanol production and its bioelectrochemical oxidation. Here, it is shown that it is possible to produce ethanol and use it as a biofuel in a tandem manner. The strategy is to employ flexible carbon fibres (FCF) electrode that could adsorb both the enzyme and the yeast cells. Glucose is used as a substrate for the yeast for the production of ethanol, while the enzyme is used to catalyse the oxidation of ethanol to acetaldehyde. Regarding the generation of reliable electricity based on electrochemical systems, the biosystem proposed in this study operates at a low temperature and ethanol production is proportional to the generated current. With further optimisation of electrode design, we envision the use of the cooperative biofuel cell for energy conversion and management of organic compounds.
将原位生物燃料生产和能量转换集成到单个系统中，可确保生成更强大的网络和更多可再生技术。为此，识别和开发新的生物催化剂至关重要。在此，我们报告了一种包含醇脱氢酶 (ADH) 和酿酒酵母的生物电化学系统，其中两者协同作用以进行乙醇生产及其生物电化学氧化。在这里，我们证明了可以串联方式生产乙醇并将其用作生物燃料。该策略是使用能够吸附酶和酵母细胞的柔性碳纤维 (FCF) 电极。葡萄糖被用作酵母生产乙醇的底物，而酶则用于催化乙醇氧化为乙醛。关于基于电化学系统的可靠电力的产生，本研究中提出的生物系统在低温下运行，并且乙醇的产量与产生的电流成正比。通过进一步优化电极设计，我们设想将协同生物燃料电池用于能量转换和有机化合物的管理。
