Karasawa Koji, Sano Yoshihiro, Kato Nobumasa, Arakawa Hidetoshi
Showa University, Tokyo, Japan.
Karasuyama Hospital, Showa University School of Medicine, Tokyo, Japan.
Luminescence. 2018 Jun;33(4):670-674. doi: 10.1002/bio.3462. Epub 2018 Mar 7.
The development of a highly sensitive analytical method for oxytocin could be useful in the diagnosis and treatment of autistic spectrum disorder. We previously developed a colorimetric enzyme immunoassay (EIA) for plasma oxytocin measurement. In this study, we developed a method to measure oxytocin concentrations using a higher sensitivity bioluminescent EIA. Biotinylated oxytocin bridged with five lysine residues was used in a competitive format. The standard curve range for oxytocin was 1.0 to 1000 pg/assay. In addition, there was good correlation between the colorimetric and bioluminescent immunoassays in terms of measured oxytocin concentration (r = 0.9665, n = 48). The bioluminescent EIA for plasma oxytocin was more rapid and provided higher sensitivity than the colorimetric immunoassay, making it suitable for clinical application.
开发一种高灵敏度的催产素分析方法可能有助于自闭症谱系障碍的诊断和治疗。我们之前开发了一种用于测量血浆催产素的比色酶免疫分析（EIA）方法。在本研究中，我们开发了一种使用更高灵敏度的生物发光EIA来测量催产素浓度的方法。用与五个赖氨酸残基桥连的生物素化催产素采用竞争法。催产素的标准曲线范围为每次测定1.0至1000 pg。此外，就测得的催产素浓度而言，比色免疫分析和生物发光免疫分析之间具有良好的相关性（r = 0.9665，n = 48）。用于血浆催产素的生物发光EIA比色免疫分析更快且灵敏度更高，使其适用于临床应用。
