Wannachod Thanaporn, Wannasutthiwat Sutthinee, Powtongsook Sorawit, Nootong Kasidit
a Chemical Engineering Research Unit for Valued-adding of Bioresource, Department of Chemical Engineering, Faculty of Engineering , Chulalongkorn University , Bangkok , Thailand.
b Center of Excellence in Marine Biotechnology, Department of Marine Science, Faculty of Science , Chulalongkorn University , Bangkok , Thailand.
Prep Biochem Biotechnol. 2018 Apr 21;48(4):335-342. doi: 10.1080/10826068.2018.1446152. Epub 2018 Mar 30.
Photoautotrophic cultivation of Chlorococcum humicola was performed in batch and continuous modes in different cultivating system arrangements to compare biomass and carotenoids' concentration and their productivities. Batch result from stirred tank and airlift photobioreactors indicated the positive effect of increasing light intensity on growth and carotenoid production, whereas the finding from continuous cultivation indicated that carotenoid enhancement preferred high light intensity and nitrogen-deficient environment. The highest biomass (1.31 ± 0.04 g L) and carotenoid (4.59 ± 0.06 mg L) concentration as well as the highest productivities, 0.46 g L d for biomass and 1.61 mg L d for carotenoids, were obtained when maintaining high light intensity of 10 klx, BG-11 medium and 2% (v/v) CO simultaneously, while the highest carotenoid content (4.84 mg g) was associated with high light intensity and nitrogen-deficient environment, which was induced by feed-modified BG-11 growth medium containing nitrate 20 folds lower than the original medium. Finally, the cultivating system arranged into smaller stirred tank photobioreactors in series yielded approximately 2.5 folds increase in both biomass and carotenoid productivities relative to using single airlift photobioreactor with equivalent working volume and similar operating condition.
在不同的培养系统配置中，以分批和连续模式对绿球藻进行光合自养培养，以比较生物量和类胡萝卜素的浓度及其生产率。搅拌罐式和气升式光生物反应器的分批培养结果表明，光照强度增加对生长和类胡萝卜素生产有积极影响，而连续培养的结果表明，类胡萝卜素的增加更倾向于高光强和缺氮环境。当同时保持10 klx的高光强、BG-11培养基和2%（v/v）的CO时，获得了最高生物量（1.31±0.04 g L）和类胡萝卜素（4.59±0.06 mg L）浓度以及最高生产率，生物量为0.46 g L d，类胡萝卜素为1.61 mg L d，而最高类胡萝卜素含量（4.84 mg g）与高光强和缺氮环境有关，这是由硝酸盐含量比原始培养基低20倍的饲料改良BG-11生长培养基诱导的。最后，相对于使用具有相同工作体积和相似操作条件的单个气升式光生物反应器，串联排列成较小搅拌罐式光生物反应器的培养系统在生物量和类胡萝卜素生产率方面均提高了约2.5倍。
