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COP1，一种光形态建成的负调控因子，通过双链 RNA 结合蛋白正向调控植物的抗病性。

COP1, a negative regulator of photomorphogenesis, positively regulates plant disease resistance via double-stranded RNA binding proteins.

机构信息

Department of Plant Pathology, University of Kentucky, Lexington, KY, United States of America.

Université de Perpignan Via Domitia, CNRS UMR5096 LGDP, Perpignan, France.

出版信息

PLoS Pathog. 2018 Mar 7;14(3):e1006894. doi: 10.1371/journal.ppat.1006894. eCollection 2018 Mar.

DOI:10.1371/journal.ppat.1006894
PMID:29513740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5871017/
Abstract

The E3 ubiquitin ligase COP1 (Constitutive Photomorphogenesis 1) is a well known component of the light-mediated plant development that acts as a repressor of photomorphogenesis. Here we show that COP1 positively regulates defense against turnip crinkle virus (TCV) and avrRPM1 bacteria by contributing to stability of resistance (R) protein HRT and RPM1, respectively. HRT and RPM1 levels and thereby pathogen resistance is significantly reduced in the cop1 mutant background. Notably, the levels of at least two double-stranded RNA binding (DRB) proteins DRB1 and DRB4 are reduced in the cop1 mutant background suggesting that COP1 affects HRT stability via its effect on the DRB proteins. Indeed, a mutation in either drb1 or drb4 resulted in degradation of HRT. In contrast to COP1, a multi-subunit E3 ligase encoded by anaphase-promoting complex (APC) 10 negatively regulates DRB4 and TCV resistance but had no effect on DRB1 levels. We propose that COP1-mediated positive regulation of HRT is dependent on a balance between COP1 and negative regulators that target DRB1 and DRB4.

摘要

E3 泛素连接酶 COP1（组成性光形态发生 1）是一种已知的光介导植物发育的组成部分，作为光形态发生的抑制剂。在这里，我们表明 COP1 通过分别促进抗性（R）蛋白 HRT 和 RPM1 的稳定性来积极调节对芜菁花叶病毒（TCV）和 avrRPM1 细菌的防御。在 cop1 突变体背景中，HRT 和 RPM1 的水平以及因此病原体抗性显著降低。值得注意的是，在 cop1 突变体背景中，至少两种双链 RNA 结合（DRB）蛋白 DRB1 和 DRB4 的水平降低，表明 COP1 通过其对 DRB 蛋白的影响影响 HRT 稳定性。事实上，在 drb1 或 drb4 中的突变导致 HRT 降解。与 COP1 相反，由后期促进复合物（APC）10 编码的多亚基 E3 连接酶负调节 DRB4 和 TCV 抗性，但对 DRB1 水平没有影响。我们提出 COP1 介导的 HRT 的正调节依赖于 COP1 和靶向 DRB1 和 DRB4 的负调节剂之间的平衡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/9e03fa5f1785/ppat.1006894.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/dd30122e555b/ppat.1006894.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/801b1cdf2efb/ppat.1006894.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/babca2f55e62/ppat.1006894.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/07719b2c25fd/ppat.1006894.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/0beadc883713/ppat.1006894.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/9e03fa5f1785/ppat.1006894.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/dd30122e555b/ppat.1006894.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/801b1cdf2efb/ppat.1006894.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/babca2f55e62/ppat.1006894.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/07719b2c25fd/ppat.1006894.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/0beadc883713/ppat.1006894.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd2b/5871017/9e03fa5f1785/ppat.1006894.g006.jpg

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