用于产生伪感染性黄热病毒颗粒的包装细胞系的开发与特性分析。
Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation.
作者信息
Queiroz Sabrina Ribeiro de Almeida, Silva Júnior José Valter Joaquim, Silva Andréa Nazaré Monteiro Rangel da, Carvalho Amanda Gomes de Oliveira, Santos Jefferson José da Silva, Gil Laura Helena Vega Gonzales
机构信息
Departamento de Virologia e Terapia Experimental, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil.
Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará-UFPA, Belém, Pará, Brasil.
出版信息
Rev Soc Bras Med Trop. 2018 Jan-Feb;51(1):66-70. doi: 10.1590/0037-8682-0220-2017.
INTRODUCTION
Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins.
METHODS
HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs.
RESULTS
The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs.
CONCLUSIONS
HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
引言
伪感染性黄热病毒颗粒（YFV-PIVs）已被用于研究疫苗和病毒包装。在此，我们报告了一种表达YFV prM/E蛋白的包装细胞系的开发。
方法
将YFV prM/E和C（84个核苷酸）基因转染至HEK293细胞中，以产生HEK293-YFV-PrM/E-opt。评估这些细胞表达异源蛋白以及包装复制子repYFV-17D-LucIRES以产生YFV-PIVs的能力。
结果
证实了prM/E蛋白的表达，并且该细胞系对复制子进行了反式包装，以回收YFV-PIVs的报告基因。
结论
HEK293-YFV-prM/E-opt的反式包装能力证明了其在生物技术方面的潜在应用。
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