Dias Graciela M, Bidault Adeline, Le Chevalier Patrick, Choquet Gwenaëlle, Der Sarkissian Clio, Orlando Ludovic, Medigue Claudine, Barbe Valerie, Mangenot Sophie, Thompson Cristiane C, Thompson Fabiano L, Jacq Annick, Pichereau Vianney, Paillard Christine
Laboratoire des Sciences de l'Environnement Marin, Université de Bretagne Occidentale, UMR 6539 UBO/Centre National de la Recherche Scientifique/IRD/Ifremer, Institut Universitaire Européen de la Mer, Plouzané, France.
Laboratório de Microbiologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Front Microbiol. 2018 Feb 19;9:227. doi: 10.3389/fmicb.2018.00227. eCollection 2018.
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, , which is also pathogenic to fish. Here we report the first assembly of the complete genome of CECT4600, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan- and core genomes and to identify putative virulence factors. The core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I restriction modification system, GGDEF domain proteins, several conjugative plasmids, and a Type IV secretion system. Future research on the coevolutionary arms race between virulence factors and host resistance mechanisms will improve our understanding of how pathogenicity develops in this emerging pathogen.
自20世纪70年代在西欧引进并养殖的菲律宾蛤仔中，褐环病（BRD）自1986年以来导致了很高的死亡率。BRD的病原体是一种革兰氏阴性细菌， ，它对鱼类也具有致病性。在此，我们报告了CECT4600完整基因组的首次组装，以及从广泛的宿主和地理范围内分离出的另外16个菌株的基因组序列。我们广泛的基因组数据集使我们能够描述病原体的泛基因组和核心基因组，并鉴定推定的毒力因子。核心基因组由3352个基因组成，包括多种潜在的毒力因子，如溶血素、转录调节因子、I型限制修饰系统、GGDEF结构域蛋白、几种接合质粒和IV型分泌系统。未来关于毒力因子与宿主抗性机制之间协同进化军备竞赛的研究，将增进我们对这种新兴病原体致病性如何发展的理解。
