Saulite Liga, Pleiko Karlis, Popena Ineta, Dapkute Dominyka, Rotomskis Ricardas, Riekstina Una
Faculty of Medicine, University of Latvia, Raina Blvd. 19, LV-1586 Riga, Latvia.
Biomedical Physics Laboratory, National Cancer Institute, P. Baublio Street 3b, LT-08406 Vilnius, Lithuania.
Beilstein J Nanotechnol. 2018 Jan 29;9:321-332. doi: 10.3762/bjnano.9.32. eCollection 2018.
We created a 3D cell co-culture model by combining nanoengineered mesenchymal stem cells (MSCs) with the metastatic breast cancer cell line MDA-MD-231 and primary breast cancer cell line MCF7 to explore the transfer of quantum dots (QDs) to cancer cells. First, the optimal conditions for high-content QD loading in MSCs were established. Then, QD uptake in breast cancer cells was assessed after 24 h in a 3D co-culture with nanoengineered MSCs. We found that incubation of MSCs with QDs in a serum-free medium provided the best accumulation results. It was found that 24 h post-labelling QDs were eliminated from MSCs. Our results demonstrate that breast cancer cells efficiently uptake QDs that are released from nanoengineered MSCs in a 3D co-culture. Moreover, the uptake is considerably enhanced in metastatic MDA-MB-231 cells compared with MCF7 primary breast cancer cells. Our findings suggest that nanoengineered MSCs could serve as a vehicle for targeted drug delivery to metastatic cancer.
我们通过将纳米工程化间充质干细胞（MSCs）与转移性乳腺癌细胞系MDA-MD-231和原发性乳腺癌细胞系MCF7相结合，创建了一个3D细胞共培养模型，以探索量子点（QDs）向癌细胞的转移。首先，确定了MSCs中高含量QD负载的最佳条件。然后，在与纳米工程化MSCs进行3D共培养24小时后，评估乳腺癌细胞对QD的摄取情况。我们发现，在无血清培养基中用QD孵育MSCs可提供最佳的积累结果。结果发现，标记后24小时，QD从MSCs中被清除。我们的结果表明，在3D共培养中，乳腺癌细胞能有效摄取从纳米工程化MSCs释放的QD。此外，与MCF7原发性乳腺癌细胞相比，转移性MDA-MB-231细胞中的摄取显著增强。我们的研究结果表明，纳米工程化MSCs可作为向转移性癌症进行靶向药物递送的载体。
