2009 年至 2015 年东南亚地区轮状病毒的分子流行病学研究。
Molecular epidemiology of rotaviruses in the south-east Asian region from 2009 to 2015.
机构信息
Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
出版信息
Vaccine. 2018 Dec 14;36(51):7851-7855. doi: 10.1016/j.vaccine.2018.02.092. Epub 2018 Mar 5.
BACKGROUND
In Asia, rotavirus accounts for approximately 45% of admissions due to acute gastroenteritis in children <5 years, and causes about 145,000 deaths every year. We studied the distribution of rotavirus strains from Myanmar, Sri Lanka, and Nepal during 2009-2015.
METHODS
Stool samples collected from children <5 years of age hospitalized with acute diarrhea in the three sites and positive for rotavirus antigen by enzyme immunoassay (EIA) were sent to the Christian Medical College, Vellore from 2009 to 2015. G and P typing of rotavirus strains were performed using reverse-transcription polymerase chain reaction (RT-PCR).
RESULT
Of the 2354 EIA positive samples tested, G12P[8] (36.8%), G1P[8] (30.1%), and G12P[6] (41.3%) were the most common strains isolated from Myanmar, Sri Lanka, and Nepal respectively.
CONCLUSION
There was substantial diversity of rotavirus genotypes, and continued surveillance in developing countries of Asia will help in understanding the epidemiology of rotavirus before and after introduction of vaccines.
背景
在亚洲，轮状病毒约占 5 岁以下儿童因急性肠胃炎住院的 45%，每年导致约 14.5 万人死亡。我们研究了 2009 年至 2015 年期间来自缅甸、斯里兰卡和尼泊尔的轮状病毒株的分布情况。
方法
从三个地点因急性腹泻住院且酶联免疫吸附试验（EIA）检测到轮状病毒抗原阳性的 5 岁以下儿童收集粪便样本，并于 2009 年至 2015 年寄往维洛尔基督教医学院。使用逆转录聚合酶链反应（RT-PCR）对轮状病毒株进行 G 和 P 型分型。
结果
在 2354 份经 EIA 检测呈阳性的样本中，从缅甸、斯里兰卡和尼泊尔分离出的最常见的轮状病毒株分别为 G12P[8]（36.8%）、G1P[8]（30.1%）和 G12P[6]（41.3%）。
结论
轮状病毒基因型存在很大的多样性，在亚洲发展中国家进行持续监测将有助于在疫苗引入前后了解轮状病毒的流行病学情况。
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