Milei J, Ferreira R, Storino R A, Barone H D, Barone S A
Arch Inst Cardiol Mex. 1987 Jan-Feb;57(1):35-40.
Ultrastructural studies were made of 7 glutaraldehyde-treated porcine valvular heterografts. Five prosthesis had been preserved for a period of 24 months. The remaining two, presented patched areas of different appearance and were eliminated at the routine control studies. Microscopic and electron transmission microscopic observations demonstrated disruption of the endothelial cell layer with preservation of the architecture in the remaining layers. This is consistent with previous works. The other two valves presented lipid degeneration of the spongiosa in one and diffuse fibrous degeneration in the other. This work demonstrates that valves submitted to prolonged storage periods do not undergo further alterations than those recently preserved. It also demonstrates that the pigs own pathology may cause significant structural modifications of the tissue which are only detected with rigorous examination using magnifying optical techniques.
对7个经戊二醛处理的猪瓣膜异种移植物进行了超微结构研究。其中5个假体保存了24个月。其余两个呈现出外观不同的修补区域，并在常规对照研究中被剔除。显微镜和电子透射显微镜观察显示内皮细胞层遭到破坏，其余各层结构保持完整。这与先前的研究结果一致。另外两个瓣膜，一个出现海绵层脂质变性，另一个出现弥漫性纤维变性。这项研究表明，经过长时间保存的瓣膜与近期保存的瓣膜相比，不会发生更多变化。它还表明，猪自身的病理状况可能导致组织发生显著的结构改变，而这些改变只有通过使用放大光学技术进行严格检查才能检测到。
