Quadram Institute Bioscience, Norwich Research Park, Norwich, England.
Spectrophon Ltd, Rehovot, Israel.
Proteomics. 2018 May;18(9):e1700447. doi: 10.1002/pmic.201700447.
Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in the State Literary-Memorial Museum-Reserve A. P. Chekhov Melikhovo (nearby Moscow), have been analyzed by applying EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C resins) diskettes to these surfaces. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectrometry. The environmental microbiota present on samples and the Mycobacterium tuberculosis strain were described by a meta-proteomics approach. Eight identified M. tuberculosis proteins confirmed the presence of the bacterium and the cause of Chekhov's death, in addition to several sequenced peptides belonging to other bacterial species. The human plasma proteins and human keratins, detected on a tiny blood spot on the shirt, demonstrated the power of the combined approach.
五种不同的信件和明信片，以及安东·契诃夫（Anton Chekhov）去世时所穿的衬衫，被保存在 A.P.契诃夫梅利霍沃国家文学纪念博物馆-保护区（莫斯科附近），这些物品的表面都已使用 EVA（一种带有压碎的强阴离子和阳离子交换剂和 C 树脂的乙烯-醋酸乙烯共聚物箔）磁盘进行了分析。三种不同的洗脱液（在酸性和碱性条件下以及用乙腈）通过高分辨率质谱进行了分析。通过元蛋白质组学方法描述了样品上存在的环境微生物组和结核分枝杆菌菌株。除了属于其他细菌物种的几个测序肽外，鉴定出的八种结核分枝杆菌蛋白证实了该细菌的存在和契诃夫死亡的原因。衬衫上的一个小血斑上检测到的人血浆蛋白和人角蛋白，证明了联合方法的强大。
