Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, 101149, China.
Souzhou 5th People's Hospital, Souzhou, 215007, China.
Sci Rep. 2018 Mar 9;8(1):4236. doi: 10.1038/s41598-018-22495-3.
We compared the positive rates of T-SPOT.TB and bacterial culture in the smear-negative PTB, and analyzed the factors affecting the results of negative T-SPOT.TB and bacterial culture. Retrospective evaluation of data from smear-negative PTB patients who underwent T-SPOT.TB and bacterial culture were done. The agreement and concordance were analyzed between T-SPOT.TB and bacterial culture. Multivariable logistic regression analysis was used to explore the factors associated with positive results of T-SPOT.TB and bacterial culture in smear-negative PTB. 858 eligible smear-negative PTB patients were included in the study. The agreement rate was 25.6% (22.7~28.5%) between T-SPOT.TB and bacterial culture in smear- negative PTB patients. The positive rate of T-SPOT.TB was higher than that of bacterial culture in smear-negative PTB patients (p < 0.001). There were nearly no concordance between T-SPOT.TB and bacterial culture (p > 0.05). Using multivariable logistic regression analysis we found that older age ≥ 60 years (OR = 0.469, 95% CI: 0.287-0.768) and decreased albumin (OR = 0.614, 95% CI: 0.380-0.992) were associated with negative diagnostic results of T-SPOT.TB in smear-negative PTB patients. Female (OR = 0.654, 95% CI: 0.431-0.992) were associated with negative diagnostic results of bacteria culture in smear-negative PTB patients. Our results indicated that the older age and decreased albumin were independently associated with negative T-SPOT.TB responses.
我们比较了 T-SPOT.TB 和涂片阴性肺结核细菌培养的阳性率，并分析了影响阴性 T-SPOT.TB 和细菌培养结果的因素。对接受 T-SPOT.TB 和细菌培养的涂片阴性肺结核患者的数据进行了回顾性评估。分析了 T-SPOT.TB 与细菌培养之间的一致性和符合率。采用多变量逻辑回归分析探讨了涂片阴性肺结核患者 T-SPOT.TB 和细菌培养阳性结果的相关因素。研究纳入了 858 例符合条件的涂片阴性肺结核患者。T-SPOT.TB 与涂片阴性肺结核患者细菌培养之间的一致性率为 25.6%（22.7%~28.5%）。T-SPOT.TB 在涂片阴性肺结核患者中的阳性率高于细菌培养（p<0.001）。T-SPOT.TB 与细菌培养之间几乎没有一致性（p>0.05）。多变量逻辑回归分析发现，年龄≥60 岁（OR=0.469，95%CI：0.287-0.768）和白蛋白降低（OR=0.614，95%CI：0.380-0.992）与涂片阴性肺结核患者 T-SPOT.TB 阴性诊断结果相关。女性（OR=0.654，95%CI：0.431-0.992）与涂片阴性肺结核患者细菌培养阴性诊断结果相关。我们的结果表明，年龄较大和白蛋白降低与 T-SPOT.TB 阴性反应独立相关。
