Vilím V, Hurych J
Connect Tissue Res. 1987;16(1):27-40. doi: 10.3109/03008208709001992.
The biosynthesis of sulfated glycosaminoglycans (GAG) was studied in healthy and fibrotic lung tissue (experimental silicosis). Male rats received a single intratracheal instillation of 25 mg quartz dust (DQ 12) in 0.7 ml saline and were sacrificed at days 15, 30, 90, 150 and 240 after the insult. GAGs were labeled in vivo by i.p. (intraperitoneal) administration of [35S] sulfate. The concentration of GAGs in delipidated dry tissue was unaffected, however, the uptake of [35S] sulfate into silicotic lung tissue was significantly higher than in controls. Identification of sulfated GAGs isolated from pooled samples by DEAE-cellulose chromatography was achieved by electrophoresis and chondroitinase digestion. The most marked change in the label distribution among individual GAGs occurred with regard to dermatan sulfate. The fraction of label in dermatan sulfate was elevated in silicotic lung.
在健康和纤维化肺组织（实验性矽肺）中研究了硫酸化糖胺聚糖（GAG）的生物合成。雄性大鼠经气管内单次注入0.7 ml盐水中的25 mg石英粉尘（DQ 12），并在损伤后第15、30、90、150和240天处死。通过腹腔内（i.p.）给予[35S]硫酸盐在体内标记GAG。脱脂干燥组织中GAG的浓度未受影响，然而，[35S]硫酸盐进入矽肺组织的摄取明显高于对照组。通过电泳和软骨素酶消化，通过DEAE-纤维素色谱法从合并样品中分离出的硫酸化GAG得以鉴定。在各个GAG之间标记分布最显著的变化发生在硫酸皮肤素方面。矽肺肺组织中硫酸皮肤素的标记部分升高。
