Khuu Alicia, Chadwick Scott, Moret Sébastien, Spindler Xanthe, Gunn Peter, Roux Claude
University of Technology Sydney (UTS), Centre for Forensic Science, P.O. Box 123, Broadway, NSW 2007, Australia.
Forensic Sci Int. 2018 May;286:1-7. doi: 10.1016/j.forsciint.2018.02.015. Epub 2018 Feb 23.
Fingermarks can be exploited for both their ridge detail and touch DNA. One-step luminescent cyanoacrylate (CA) fuming techniques used for fingermark enhancement, such as PolyCyano UV (Foster+Freeman Ltd) and Lumicyano™ (Crime Science Technology), claim to be compatible with DNA analysis as they reduce the need for post-staining to increase contrast of the developed fingermark. The aim of this study was to determine the impact that these one-step luminescent cyanoacrylates have on DNA analysis and how they compare to conventional CA techniques. Four donors each deposited five sets of natural fingermarks, to which a known amount of washed saliva cells was dispensed onto half of each set of fingermarks. Each set was treated with either a conventional CA technique or a one-step luminescent CA technique prior to collection and processing of DNA, with one set left as a non-fumed control. It was found that DNA was still recoverable and detectable following each of the treatments. Lumicyano™ had a similar impact on DNA profiles as conventional CA fuming and with post-stain, however, the degradation effect of PolyCyano UV on DNA was greater than the conventional treatments. For quantities of DNA such as that from touch DNA, the use of PolyCyano UV to enhance fingermarks may impact subsequent DNA analysis by causing allele drop out at larger fragment sizes.
指纹可因其纹路细节和接触性DNA而被利用。用于增强指纹的一步法发光氰基丙烯酸酯（CA）熏蒸技术，如PolyCyano UV（福斯特+弗里曼有限公司）和Lumicyano™（犯罪科学技术公司），声称与DNA分析兼容，因为它们减少了染色后处理以增加显影指纹对比度的需求。本研究的目的是确定这些一步法发光氰基丙烯酸酯对DNA分析的影响，以及它们与传统CA技术的比较情况。四名捐赠者每人留下五组自然指纹，在每组指纹的一半上滴加已知量的洗涤后的唾液细胞。在收集和处理DNA之前，每组指纹分别用传统CA技术或一步法发光CA技术处理，一组作为未熏蒸的对照。结果发现，每种处理后DNA仍可回收并检测到。Lumicyano™对DNA图谱的影响与传统CA熏蒸及染色后处理相似，然而，PolyCyano UV对DNA的降解作用大于传统处理。对于接触性DNA等数量的DNA，使用PolyCyano UV增强指纹可能会导致较大片段大小的等位基因缺失，从而影响后续的DNA分析。
