McIntosh T K
Endocrinology. 1987 May;120(5):1734-41. doi: 10.1210/endo-120-5-1734.
Recent evidence has suggested that a circadian rhythm exists for plasma beta-endorphin-like immunoreactivity. The purpose of the present study was to examine the long term effects of surgical trauma on plasma beta-endorphin dynamics. Blood samples for RIA were obtained from female baboons every 4 h for three 48-h periods: one beginning 1 week before surgical trauma, the second 30 min after surgical trauma, and the third 1 week after surgical trauma. Animals were subjected to laparotomy and 30-min anesthesia (n = 8), 5-min surgical trauma under 30-min anesthesia (low trauma; n = 8), or 20-min surgical trauma under 30-min anesthesia (high trauma; n = 8). Computer analysis of beta-endorphin levels as a function of clock time demonstrated a true preoperative circadian rhythm for all animals, with a mean of 87.9 pg/ml. In the immediate 48-h postoperative period, a postoperative alteration in circadian beta-endorphin dynamics occurred that was correlated with the severity of trauma. A disruption of circadian rhythms of plasma beta-endorphin occurred in the high trauma group only, in which it persisted for longer than 1 week after trauma. These studies establish a relationship between the alteration of circadian rhythmicity of plasma beta-endorphin-like immunoreactivity and the magnitude of trauma and injury.
最近有证据表明，血浆β-内啡肽样免疫反应存在昼夜节律。本研究的目的是探讨手术创伤对血浆β-内啡肽动态变化的长期影响。在三个48小时时间段内，每4小时从雌性狒狒身上采集用于放射免疫分析（RIA）的血样：一个时间段始于手术创伤前1周，第二个时间段始于手术创伤后30分钟，第三个时间段始于手术创伤后1周。对动物进行剖腹术和30分钟麻醉（n = 8）、在30分钟麻醉下进行5分钟手术创伤（轻度创伤；n = 8）或在30分钟麻醉下进行20分钟手术创伤（重度创伤；n = 8）。对β-内啡肽水平作为时钟时间函数的计算机分析表明，所有动物术前均存在真正的昼夜节律，平均值为87.9 pg/ml。在术后立即的48小时内，昼夜β-内啡肽动态发生了术后改变，这与创伤的严重程度相关。只有重度创伤组出现了血浆β-内啡肽昼夜节律的紊乱，且在创伤后持续超过1周。这些研究确立了血浆β-内啡肽样免疫反应昼夜节律改变与创伤和损伤程度之间的关系。
