Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil.
School of Civil Engineering & Geosciences, Newcastle University, NE1 7RU Newcastle upon Tyne, UK.
Bioresour Technol. 2018 Jun;258:208-219. doi: 10.1016/j.biortech.2018.02.083. Epub 2018 Feb 23.
The effects of temperature reduction (from 35 °C to 20 °C) on nitrogen removal performance and microbial diversity of an anammox sequencing batch reactor were evaluated. The reactor was fed for 148 days with anaerobically pretreated municipal wastewater amended with nitrite. On average, removal efficiencies of ammonium and nitrite were high (96%) during the enrichment period and phases 1 (at 35 °C) and 2 (at 25 °C), and slightly decreased (to 90%) when the reactor was operated at 20 °C. Deep sequencing analysis revealed that microbial community structure changed with temperature decrease. Anammox bacteria (Ca. Brocadia and Ca. Anammoximicrobium) and denitrifiers (Burkholderiales, Myxococcales, Rhodocyclales, Xanthomonadales, and Pseudomonadales) were favoured when the temperature was lowered from 35 °C to 25 °C, while Anaerolineales and Clostridiales were negatively affected. The results support the feasibility of using the anammox process for mainstream nitrogen removal from anaerobically pretreated municipal wastewater at typical tropical temperatures.
评估了温度降低（从 35°C 降至 20°C）对厌氧预处理城市污水中添加亚硝酸盐的厌氧氨氧化序批式反应器脱氮性能和微生物多样性的影响。该反应器在富集期以及第 1 阶段（35°C）和第 2 阶段（25°C）期间，以高平均去除效率（96%）处理了铵和亚硝酸盐，当反应器在 20°C 下运行时，去除效率略有下降（至 90%）。深度测序分析表明，微生物群落结构随温度降低而变化。当温度从 35°C 降低到 25°C 时，厌氧氨氧化菌（Ca. Brocadia 和 Ca. Anammoximicrobium）和反硝化菌（Burkholderiales、Myxococcales、Rhodocyclales、Xanthomonadales 和 Pseudomonadales）受到青睐，而 Anaerolineales 和 Clostridiales 则受到负面影响。结果支持了在典型热带温度下，使用厌氧氨氧化工艺从厌氧预处理城市废水中去除主流氮的可行性。
