Delory Benjamin M, Weidlich Emanuela W A, van Duijnen Richard, Pagès Loïc, Temperton Vicky M
Ecosystem Functioning and Services, Institute of Ecology, Leuphana University, Lüneburg, Germany.
INRA, Centre PACA, UR 1115 PSH, Avignon Cedex 9, France.
Methods Mol Biol. 2018;1761:3-22. doi: 10.1007/978-1-4939-7747-5_1.
In this chapter, we present methods that we routinely use to measure plant root traits in the field and under controlled environmental conditions (using rhizoboxes). We describe procedures to (1) collect, wash, and store root samples, (2) acquire images of washed root samples, and (3) measure root traits using image analysis. In addition, we also describe sampling methods for studying belowground productivity, soil exploration, and root distribution in the first soil layers at the community level (soil coring and ingrowth core method). Because the use of rhizoboxes allows a nondestructive and dynamic measurement of traits hardly accessible in the field, a section of this chapter is devoted to the acquisition and analysis of images of roots growing in rhizoboxes.
在本章中，我们介绍了我们通常用于在田间和受控环境条件下（使用根箱）测量植物根系性状的方法。我们描述了以下步骤：(1) 采集、清洗和储存根系样本；(2) 获取清洗后的根系样本图像；(3) 使用图像分析测量根系性状。此外，我们还描述了在群落水平上研究地下生产力、土壤探索和第一土壤层根系分布的采样方法（土壤取芯和内生长芯法）。由于使用根箱可以对田间难以获取的性状进行无损动态测量，因此本章有一部分专门介绍根箱中生长根系图像的获取和分析。
