Schöller Maria, Sarkel Elizabeth, Kleine-Vehn Jürgen, Feraru Elena
Department of Applied Genetics and Cell Biology (DAGZ), University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, Vienna, 1190, Austria.
Methods Mol Biol. 2018;1761:199-208. doi: 10.1007/978-1-4939-7747-5_15.
Time-lapse imaging of roots is highly suitable for depicting gravitropic growth behaviors. However, roots may show faster or slower bending kinetics when compared to control as a result of differences in overall root growth. Accordingly, conditions that cause differential organ growth require growth rate normalization to compare gravitropic curvature. Here, we describe a simple normalization method for gravitropic root growth evaluation. We exemplify this method by exposing seedlings to distinct environmental conditions or disturbing the cellular auxin contents. This data shows that the method is suitable to discriminate between gravitropic and overall organ growth defects.
对根进行延时成像非常适合描绘向重力性生长行为。然而，由于整体根生长存在差异，与对照相比，根可能表现出更快或更慢的弯曲动力学。因此，导致器官生长差异的条件需要进行生长速率归一化处理，以便比较向重力性曲率。在此，我们描述一种用于评估向重力性根生长的简单归一化方法。我们通过将幼苗置于不同环境条件下或干扰细胞生长素含量来举例说明该方法。这些数据表明该方法适用于区分向重力性和整体器官生长缺陷。
