Cortijo Sandra, Charoensawan Varodom, Roudier François, Wigge Philip A
The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Methods Mol Biol. 2018;1761:231-248. doi: 10.1007/978-1-4939-7747-5_18.
Chromatin immunoprecipitation combined with next-generation sequencing (ChIP-seq) is a powerful technique to investigate in vivo transcription factor (TF) binding to DNA, as well as chromatin marks. Here we provide a detailed protocol for all the key steps to perform ChIP-seq in Arabidopsis thaliana roots, also working on other A. thaliana tissues and in most non-ligneous plants. We detail all steps from material collection, fixation, chromatin preparation, immunoprecipitation, library preparation, and finally computational analysis based on a combination of publicly available tools.
染色质免疫沉淀结合新一代测序（ChIP-seq）是一种用于研究体内转录因子（TF）与DNA结合以及染色质标记的强大技术。在此，我们提供了一套详细的方案，涵盖了在拟南芥根中进行ChIP-seq的所有关键步骤，该方案也适用于拟南芥的其他组织以及大多数非木质植物。我们详细介绍了从材料收集、固定、染色质制备、免疫沉淀、文库制备，到最终基于多种公开可用工具组合进行计算分析的所有步骤。
