Maacha Selma, Saule Simon
Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
Unité Mixte de Recherche 3347 (UMR), Unité 1021, Centre National de La Recherche Scientifique (CNRS), Institut National de la Santé Et de la Recherche Médicale (INSERM), Institut Curie, PSL Research University, Orsay, France.
Methods Mol Biol. 2018;1749:71-77. doi: 10.1007/978-1-4939-7701-7_8.
Metastases is largely responsible for the mortality among cancer patients. Metastasis formation is a complex multistep process, which results from the propagation of cancer cells from the primary tumor to distant sites of the body. Research on cancer metastasis aims to understand the mechanisms involved in the spread of cancer cells through the development of in vivo assays that assess cell invasion. Here we describe the use of the chick chorioallantoic membrane to evaluate cancer cell invasiveness in vivo. The chick chorioallantoic membrane assay is based on the detection and quantification of disseminated human tumor cells in the chick embryo femurs by real-time PCR amplification of human Alu sequences.
转移在很大程度上导致了癌症患者的死亡。转移灶的形成是一个复杂的多步骤过程，它源于癌细胞从原发性肿瘤扩散到身体的远处部位。癌症转移的研究旨在通过开发评估细胞侵袭的体内试验来了解癌细胞扩散所涉及的机制。在此，我们描述了利用鸡胚绒毛尿囊膜在体内评估癌细胞侵袭性的方法。鸡胚绒毛尿囊膜试验基于通过对人Alu序列进行实时PCR扩增来检测和定量鸡胚股骨中播散的人肿瘤细胞。
