鸡胚尿囊膜（CAM）模型作为一种体内方法用于研究恶性肿瘤细胞的侵袭和转移级联。
The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells.
机构信息
Department of Experimental Surgery - Cancer Metastasis, Medical Faculty Mannheim, Ruprecht Karls University of Heidelberg, Mannheim, Germany.
Centre for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Ruprecht Karls University of Heidelberg, Mannheim, Germany.
出版信息
Methods Mol Biol. 2021;2294:17-26. doi: 10.1007/978-1-0716-1350-4_2.
The CAM model enables an in vivo analysis of the individual sub-steps of the metastatic cascade like local invasion, intravasation, or the establishment of metastasis in particular organs. Incubated fertilized chicken eggs are inoculated with human tumor cells and further processed for up to 9-10 days. The invasion and metastasis of these cells is then detected quantitatively with high specificity and sensitivity by means of a PCR for human ALU sequences, using the genomic DNA isolated from distant portions of the CAM, as well as from diverse internal organs of the developing embryo.
CAM 模型能够对转移级联的各个子步骤（如局部浸润、入血或在特定器官中建立转移）进行体内分析。将受精鸡胚孵育后接种人肿瘤细胞，并进一步处理 9-10 天。然后，通过使用从 CAM 远处部分以及发育胚胎的各种内部器官分离的基因组 DNA 进行的人 ALU 序列 PCR，以高特异性和灵敏度定量检测这些细胞的侵袭和转移。
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