关于小梁网细胞分离、鉴定和培养的共识建议。
Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.
机构信息
Oregon Health and Science University, United States.
University of Miami, United States.
出版信息
Exp Eye Res. 2018 Jun;171:164-173. doi: 10.1016/j.exer.2018.03.001. Epub 2018 Mar 9.
Abstract
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
摘要
培养的小梁网（TM）细胞是研究参与常规流出阻力调节和眼内压的细胞机制的有价值的模型系统；其功能障碍导致眼高压。在这篇综述中，我们描述了从包括人类在内的几种动物物种中分离 TM 细胞的标准程序，以及用于验证其身份的方法。当用作模型时，具有一套 TM 细胞的标准操作程序将提高科学严谨性，并使其他研究人员能够复制和建立在以前的发现基础上。
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