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从农田中分离出用于降解丁草胺的探矿菌JF菌株。

Prospecting sp. JF isolated from agricultural fields for butachlor degradation.

作者信息

Singh Jatinder, Kadapakkam Nandabalan Yogalakshmi

机构信息

Centre for Environmental Science and Technology, School of Environment and Earth Sciences, Central University of Punjab, Bathinda, Punjab 151001 India.

出版信息

3 Biotech. 2018 Mar;8(3):164. doi: 10.1007/s13205-018-1165-7. Epub 2018 Mar 7.

DOI:10.1007/s13205-018-1165-7
PMID:29527451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5840082/
Abstract

Butachlor is a chloroacetamide herbicide used worldwide for controlling weeds in plants of rice, corn, soybean and other crops. In this study, indigenous bacterial species sp. JF was isolated from the agricultural fields of Punjab and identified using 16S ribosomal RNA analysis. The bacteria utilized butachlor as the sole carbon source and showed complete degradation (100 mg/L) within 24 h of incubation. Two intermediate products, namely 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester and 2,4-bis(1,1-dimethylethyl)-phenol were observed at the end of butachlor degradation. To the best of author's knowledge, biodegradation of butachlor by indigenous sp. JF from the agricultural fields of Punjab has not been reported so far.

摘要

丁草胺是一种氯乙酰胺类除草剂,在全球范围内用于控制水稻、玉米、大豆和其他作物中的杂草。在本研究中,从旁遮普邦的农田中分离出本地细菌菌株JF,并通过16S核糖体RNA分析进行鉴定。该细菌利用丁草胺作为唯一碳源,在培养24小时内显示出完全降解(100毫克/升)。在丁草胺降解结束时观察到两种中间产物,即1,2-苯二甲酸双(2-甲基丙基)酯和2,4-双(1,1-二甲基乙基)苯酚。据作者所知,旁遮普邦农田中本地菌株JF对丁草胺的生物降解迄今尚未见报道。

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本文引用的文献

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Potential Use of Rice Field Cyanobacterium Nostoc muscorum in the Evaluation of Butachlor Induced Toxicity and their Degradation.稻田蓝藻念珠藻在丁草胺诱导毒性评估及其降解中的潜在应用
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FEMS Microbiol Lett. 2013 Jul;344(2):114-20. doi: 10.1111/1574-6968.12163. Epub 2013 May 28.
4
Biodegradation of butachlor by Rhodococcus sp. strain B1 and purification of its hydrolase (ChlH) responsible for N-dealkylation of chloroacetamide herbicides.棒状杆菌属 B1 菌株对丁草胺的生物降解及其负责氯乙酰胺类除草剂 N-脱烷基化的水解酶(ChlH)的纯化。
J Agric Food Chem. 2012 Dec 19;60(50):12238-44. doi: 10.1021/jf303936j. Epub 2012 Dec 5.
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Lett Appl Microbiol. 2010 Jul;51(1):54-60. doi: 10.1111/j.1472-765X.2010.02854.x. Epub 2010 Apr 20.