Department of Microbiology, Field Marshal K. M. Cariappa College, A Constituent College of Mangalore University, Madikeri, Kodagu, Karnataka, India.
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
J Appl Microbiol. 2018 Jul;125(1):148-158. doi: 10.1111/jam.13756. Epub 2018 Apr 23.
This research aims to characterize the pigment produced by isolated fungi and to evaluate its anticancer activities.
Pigment-producing fungi was isolated and identified as Fusarium chlamydosporum. The pigment was extracted with chloroform, purified by preparative TLC and characterized by Fourier transmittance infrared, electron spray ionization mass spectroscopy, liquid chromatography mass spectroscopy and nuclear magnetic resonance (NMR) ( HNMR, C NMR) spectral analysis, which revealed the pigment to be 'long chain hydrocarbons with poly unsaturated groups' (m/z 702). Pigment stability varied with different pH, temperature and sunlight conditions. The pigment-induced cell death in human breast adenocarcinoma cells MCF-7 and showed no significant toxicity in CHOK 1 cells. Lipid peroxidation assay revealed that treatment with pigment was able to reduce the lipid peroxidation caused by H O in MCF-7 cells.
The F. chlamydosporum pigment is a compound of long-chain hydrocarbons with poly unsaturated groups, possessing selective cytotoxicity in MCF-7 cancer cell lines.
The pigment can be used as a colouring agent in cosmetics. Its anticancer potential can be used in production of therapeutics in increasing demand cancer research.
本研究旨在对分离真菌产生的色素进行表征，并评估其抗癌活性。
从真菌中分离并鉴定出产生色素的真菌为串珠镰孢菌。用氯仿提取色素，用制备薄层色谱法进行纯化，并通过傅里叶变换红外、电子喷雾电离质谱、液质联用和核磁共振（HNMR、C NMR）光谱分析进行表征，结果表明该色素为“长链多不饱和基团的碳氢化合物”（m/z 702）。色素的稳定性随 pH 值、温度和阳光条件的不同而变化。色素诱导人乳腺癌细胞 MCF-7 死亡，对 CHOK1 细胞无明显毒性。脂质过氧化测定表明，色素处理能够减少 MCF-7 细胞中 H2O2 引起的脂质过氧化。
串珠镰孢菌色素是一种长链多不饱和基团的碳氢化合物，对 MCF-7 癌细胞系具有选择性细胞毒性。
该色素可用作化妆品的着色剂。其抗癌潜力可用于日益增长的癌症研究治疗药物的生产。
