Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Chem Commun (Camb). 2018 Mar 27;54(26):3223-3226. doi: 10.1039/c8cc00838h.
We present a novel strategy for the discrimination of single nucleotide variants (SNVs) by combining cooperativity with sequestration, which displays remarkably high specificity (discrimination factors ranging from 67 to 618 with a median of 194) against 12 model SNVs and can be easily integrated with PCR amplification to detect KRAS G12D mutation.
我们提出了一种通过结合协同作用和隔离来区分单核苷酸变体 (SNV) 的新策略，该策略对 12 种模型 SNV 具有极高的特异性（区分因子范围为 67 至 618，中位数为 194），并且可以与 PCR 扩增轻松集成以检测 KRAS G12D 突变。
