Abdel-Latif Randa S, ELBadawy Nissreen E, Ibrahim Basem M
Department of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Ophthalmology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Egypt J Immunol. 2017 Jun;24(2):127-140.
This is a prospective interventional comparative study which aimed to investigate correlation between tear film allergen specific IgE levels and the skin prick test in diagnosing different types of allergic conjunctivitis. One hundred twenty patients with allergic conjunctivitis were included in this study and were classified into 4 groups based on the type of allergic conjunctivitis. Patients were subjected to skin prick test (SPT). Micro capillary method was used to collect tear samples for the quantitative assessment of specific IgE by Immune blot assay. The most common allergens were mixed mould, mixed pollen, and mixed mite. The results of tear film specific IgE in detection of allergens were evaluated against the SPT. The Receiving Operating Characteristic Curve (ROCs) revealed that tear film allergen-specific IgE specificity was 100% and sensitivity was 75%-100% to the three common allergens in the 4 studied groups. The correlation between tear's specific IgE and skin prick test was statistically significant for pollen, mite, and mould allergens in patient with SAK (r = 0.821, P ˂ 0.001 for pollen, r = 0.964, P ˂ 0.001 for mite, and r= 0.811, P ˂ 0.02 for mould ), PAC (r = 0.851, P ˂ 0.001 for pollen, r = 0.826, P ˂ 0.001 for mite, and r= 0.861, P ˂ 0.001 for mould) and VKC (r = 0.802, P ˂ 0.001 for pollen, r = 0.894, P ˂ 0.001 for mite, and r= 0.861, P ˂ 0.061 for mould). In patient with AKC, the correlation was statistically significant for only mite allergen (r = 1, P ˂ 0.001). We concluded that Tear film specific IgE test can be considered as a good alternative to skin prick test in diagnosis of the causative allergens in allergic conjunctivitis.
这是一项前瞻性干预性比较研究，旨在探讨泪膜变应原特异性IgE水平与皮肤点刺试验在诊断不同类型过敏性结膜炎中的相关性。本研究纳入了120例过敏性结膜炎患者，并根据过敏性结膜炎的类型将其分为4组。对患者进行皮肤点刺试验（SPT）。采用微量毛细管法收集泪液样本，通过免疫印迹法对特异性IgE进行定量评估。最常见的变应原是混合霉菌、混合花粉和混合螨。将泪膜特异性IgE检测变应原的结果与皮肤点刺试验结果进行对比评估。受试者工作特征曲线（ROCs）显示，在4个研究组中，泪膜变应原特异性IgE对三种常见变应原的特异性为100%，敏感性为75%-100%。在季节性过敏性结膜炎（SAK）患者中，泪液特异性IgE与皮肤点刺试验之间对于花粉、螨和霉菌变应原的相关性具有统计学意义（花粉：r = 0.821，P ˂ 0.001；螨：r = 0.964，P ˂ 0.001；霉菌：r = 0.811，P ˂ 0.02），在常年性过敏性结膜炎（PAC）患者中（花粉：r = 0.851，P ˂ 0.001；螨：r = 0.826，P ˂ 0.001；霉菌：r = 0.861，P ˂ 0.001）以及在春季角结膜炎（VKC）患者中（花粉：r = 0.802，P ˂ 0.001；螨：r = 0.894，P ˂ 0.001；霉菌：r = 0.861，P ˂ 0.061）也是如此。在特应性角结膜炎（AKC）患者中，仅螨变应原的相关性具有统计学意义（r = 1，P ˂ 0.001）。我们得出结论，在诊断过敏性结膜炎的致病变应原时，泪膜特异性IgE检测可被视为皮肤点刺试验的良好替代方法。
