College of Sciences, China Agricultural University, Beijing, 100193, China.
College of Sciences, China Agricultural University, Beijing, 100193, China.
Aquat Toxicol. 2018 May;198:129-140. doi: 10.1016/j.aquatox.2018.02.023. Epub 2018 Mar 6.
Previous study indicated that azoxystrobin had high acute toxicity to zebrafish, and larval zebrafish were more sensitive to azoxystrobin than adult zebrafish. The objective of the present study was to investigate short-term developmental effects and potential mechanisms of azoxystrobin in larval and adult zebrafish. After zebrafish embryos and adults were exposed to 0.01, 0.05 and 0.20 mg/L azoxystrobin (equal to 25, 124 and 496 nM azoxystrobin, respectively) for 8 days, the lethal effect, physiological responses, liver histology, mitochondrial ultrastructure, and expression alteration of genes related to mitochondrial respiration, oxidative stress, cell apoptosis and innate immune response were determined. The results showed that there was no significant effect on larval and adult zebrafish after exposure to 0.01 mg/L azoxystrobin. However, increased ROS, MDA concentration and il1b in larval zebrafish, as well as increased il1b, il8 and cxcl-c1c in adult zebrafish were induced after exposure to 0.05 mg/L azoxystrobin. Reduced mitochondrial complex III activity and ATP concentration, increased SOD activity, ROS and MDA concentration, decreased cytb, as well as increased sod1, sod2, cat, il1b, il8 and cxcl-c1c were observed both in larval and adult zebrafish after exposure to 0.20 mg/L azoxystrobin; meanwhile, increased p53, bax, apaf1 and casp9, alteration of liver histology and mitochondrial ultrastructure in larval zebrafish, and alteration of mitochondrial ultrastructure in adult zebrafish were also induced. The results demonstrated that azoxytrobin induced short-term developmental effects on larval zebrafish and adult zebrafish, including mitochondrial dysfunction, oxidative stress, cell apoptosis and innate immune response. Statistical analysis indicated that azoxystrobin induced more negative effects on larval zebrafish, which might be the reason for the differences of developmental toxicity between larval and adult zebrafish caused by azoxystrobin. These results provided a new insight into potential mechanisms of azoxystrobin in larval zebrafish and adult zebrafish.
先前的研究表明，唑菌酯对斑马鱼具有高急性毒性，且幼鱼比成鱼对唑菌酯更为敏感。本研究旨在探究唑菌酯对幼鱼和成鱼的短期发育毒性及潜在作用机制。将斑马鱼胚胎和成鱼暴露于 0.01、0.05 和 0.20mg/L 唑菌酯（分别相当于 25、124 和 496nM 唑菌酯）中 8 天，测定其致死效应、生理反应、肝脏组织学变化、线粒体超微结构以及与线粒体呼吸、氧化应激、细胞凋亡和固有免疫反应相关基因的表达变化。结果表明，暴露于 0.01mg/L 唑菌酯对幼鱼和成鱼均无显著影响。然而，暴露于 0.05mg/L 唑菌酯可诱导幼鱼 ROS、MDA 浓度和 il1b 增加，以及成鱼 il1b、il8 和 cxcl-c1c 增加。暴露于 0.20mg/L 唑菌酯可导致幼鱼和成鱼的线粒体复合物 III 活性和 ATP 浓度降低，SOD 活性、ROS 和 MDA 浓度升高，cytb 减少，同时 sod1、sod2、cat、il1b、il8 和 cxcl-c1c 表达增加；此外，还可引起幼鱼 p53、bax、apaf1 和 casp9 增加、肝脏组织学和线粒体超微结构改变，以及成鱼线粒体超微结构改变。这些结果表明，唑菌酯可诱导幼鱼和成鱼产生短期发育毒性，包括线粒体功能障碍、氧化应激、细胞凋亡和固有免疫反应。统计分析表明，唑菌酯对幼鱼的负面影响更大，这可能是唑菌酯引起幼鱼和成鱼发育毒性差异的原因。这些结果为唑菌酯在幼鱼和成鱼中的潜在作用机制提供了新的见解。
