双歧杆菌对坏死性小肠结肠炎新生大鼠肠道组织中β-防御素-2表达的影响
[Effect of Bifidobacterium on the expression of β-defensin-2 in intestinal tissue of neonatal rats with necrotizing enterocolitis].
作者信息
Lu Wei-Cheng, Zheng Xu, Liu Jin-Fu, Wu Wen-Chuan, Chen Xing-Yue, Wei Hai-Bo, Li Chun-Lei, Lin Ming-Jing
机构信息
Department of Neonatology, Hainan Provincial People's Hospital, Haikou 570311, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2018 Mar;20(3):224-229. doi: 10.7499/j.issn.1008-8830.2018.03.012.
OBJECTIVE
To study the effect of Bifidobacterium on the expression of β-defensin-2 (BD-2) in intestinal tissue of neonatal rats with necrotizing enterocolitis (NEC).
METHODS
A total of 40 rats were randomly divided into four groups: normal control, Bifidobacterium control, NEC model, and Bifidobacterium treatment, with 10 rats in each group. A rat model of NEC was induced by hypoxia, cold stimulation, and artificial feeding. The rats in the Bifidobacterium control and Bifidobacterium treatment groups were given Bifidobacterium via the gastric tube after cold stimulation once a day for three consecutive days. The morphological changes of the terminal ileum were observed under a light microscope and the intestinal injury score was determined. Immunohistochemistry and qRT-PCR were used to measure the protein and mRNA expression of BD-2 in the ileal mucosal tissue.
RESULTS
The NEC model group had a significantly higher intestinal injury score than the normal control, Bifidobacterium control, and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium treatment group had a significantly higher intestinal injury score than the normal control and Bifidobacterium control groups (P<0.05). The mRNA and protein expression of BD-2 in the normal control group was significantly lower than in the Bifidobacterium control, NEC model, and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium control group had significantly higher mRNA and protein expression of BD-2 than the NEC model and Bifidobacterium treatment groups (P<0.05). The Bifidobacterium treatment group had significantly higher mRNA and protein expression of BD-2 than the NEC model group (P<0.05).
CONCLUSIONS
Bifidobacterium can induce the expression of BD-2 in intestinal tissue of rats and reduce inflammatory response by increasing the expression of BD-2. This provides a protective effect on neonatal rats with NEC.
目的
研究双歧杆菌对坏死性小肠结肠炎（NEC）新生大鼠肠道组织中β-防御素-2（BD-2）表达的影响。
方法
将40只大鼠随机分为四组：正常对照组、双歧杆菌对照组、NEC模型组和双歧杆菌治疗组，每组10只。通过缺氧、冷刺激和人工喂养诱导建立NEC大鼠模型。双歧杆菌对照组和双歧杆菌治疗组的大鼠在冷刺激后经胃管给予双歧杆菌，每天1次，连续3天。在光学显微镜下观察回肠末端的形态变化并确定肠道损伤评分。采用免疫组织化学和qRT-PCR检测回肠黏膜组织中BD-2的蛋白和mRNA表达。
结果
NEC模型组的肠道损伤评分显著高于正常对照组、双歧杆菌对照组和双歧杆菌治疗组（P<0.05）。双歧杆菌治疗组的肠道损伤评分显著高于正常对照组和双歧杆菌对照组（P<0.05）。正常对照组中BD-2的mRNA和蛋白表达显著低于双歧杆菌对照组、NEC模型组和双歧杆菌治疗组（P<0.05）。双歧杆菌对照组中BD-2的mRNA和蛋白表达显著高于NEC模型组和双歧杆菌治疗组（P<0.05）。双歧杆菌治疗组中BD-2的mRNA和蛋白表达显著高于NEC模型组（P<0.05）。
结论
双歧杆菌可诱导大鼠肠道组织中BD-2的表达，并通过增加BD-2的表达减轻炎症反应。这对患有NEC的新生大鼠具有保护作用。
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