College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
Food Chem Toxicol. 2018 May;115:198-204. doi: 10.1016/j.fct.2018.03.007. Epub 2018 Mar 9.
Bovine mastitis is an infectious disease of the mammary gland which has been generally treated by antibiotic delivery. While the increasing drug-resistant bacteria and the high consumption of the antibiotic had become a noticeable concern. In a previous study, a mammary special vector expressing human β-defensin 3 (hBD3) was transfected into bovine fetal fibroblasts to produce mastitis-resistant bovine. This investigation focused on potential unintended effects of transgenic milk containing hBD3 produced by these mastitis-resistant bovine on the reproductive system of C57BL/6J mice. Mice were fed with diets containing transgenic milk or conventional milk, nutritionally balanced to an AIN93G diet for 90 days, and non-milk diet was selected as the negative group. The reproductive system was given special attention including reproductive organ/body ratios, necropsy and histopathology, serum sex hormone, sperm parameters, estrus cycle and the expression level of some specific genes which could indicate the development and function of reproductive system. No diet-related significant differences were observed among three groups in this 90-day feeding study. The results indicated that hBD3 milk does not appear to exert any effect on the reproductive system in C57BL/6J rats compared with conventional milk or the control diet.
奶牛乳腺炎是一种乳腺传染病，通常采用抗生素治疗。然而，抗生素耐药菌的不断增加和抗生素的高消耗已成为一个值得关注的问题。在之前的研究中，通过转染表达人β防御素 3（hBD3）的乳腺特异性载体，将其导入牛胎儿成纤维细胞中，从而生产出抗乳腺炎的奶牛。本研究关注的是这些抗乳腺炎奶牛所产含 hBD3 的转基因奶对 C57BL/6J 小鼠生殖系统的潜在不良影响。用含有转基因奶或常规奶的饮食喂养小鼠 90 天，这些饮食的营养与 AIN93G 饮食相平衡，而非奶饮食作为阴性对照组。生殖系统受到特别关注，包括生殖器官/体重比、尸检和组织病理学、血清性激素、精子参数、发情周期以及一些特定基因的表达水平，这些基因可以表明生殖系统的发育和功能。在这项为期 90 天的喂养研究中，三组之间没有观察到与饮食相关的显著差异。结果表明，与常规奶或对照饮食相比，hBD3 奶似乎不会对 C57BL/6J 大鼠的生殖系统产生任何影响。
