Tan Ming-Sheng, Wang Yan-Lei, Qi Ying-Na, Dong Chun-Ke, Wang Wei, Yi Ping, Yang Feng, Tang Xiang-Sheng, Hao Qing-Ying
China-Japan Friendship Hospital, Beijing 100029, China;
Zhongguo Gu Shang. 2018 Jan 25;31(1):67-73. doi: 10.3969/j.issn.1003-0034.2018.01.012.
To explore the effect and underlying mechanism of decompression(DE)combined with Governor Vessel(GV)electro-acupuncture(EA) on rats with acute severe upper cervical spinal cord compression injury.
Thirty SPF rats were randomly divided into 5 groups(control group A, B and experiment group C, D, E), 6 rats in each group. The model of acute severe upper cervical spinal cord compression injury were made by forcing a balloon catheter put in atlas pillow clearance. The group A was blank one, the group B put balloon catheter in atlas pillow clearance without forcing, and the group C, D, E sustained compressed for 48 h. The group C received electric acupuncture intervention, selecting the Baihui and Dazhui point, having the continuous wave and frequency of 2 Hz, with the treatment time of 15 min and continuous treatment for 14 d; the group D received methylprednisolone intervention, injected by caudal vein; the group E did not received any intervention again. The arterial blood and injured spinal cord tissue of all the rats were obtained after 14 days' treatment, and BBB score was used to evaluate the change of each group hind limbs motor function, the contents of platelet activating factor(PAF) in injured spinal cord tissue and blood serum were assess by ELISA method; the Caspase-9 expression for each group after 14 days' treatment was assess by Western blot method.
BBB scores were(21.000±0.000) points at the 6 time points, that was, 1 h, 48 h after forcing in control group, 24 h, 3 d, 7 d, 14 d after treating in experiment group; the score of experimental groups (group C, D, E) were always lower than control groups(group A, B); compared with group E, group C and D were significantly higher(<0.05); and there was no significant difference between group C and group D(>0.05). The results of PAF by ELISA method to measure:the concentration of serum PAF, there was no statistical difference among group A, B, D, E (>0.05), group C was lower than the other groups (<0.05); the concentration of tissue PAF, there was no significant difference between group A and group B(>0.05), group D was significantly higher than that of group A, B, and C(<0.05), group E was the highest one than that of the other groups(<0.05). Western blot med tests showed that the Caspase-9 protein expression in group A and B was similar (>0.05), group C was higher than that of group A and B(<0.05), group D was higher than group A, B and C(<0.05), group E was the highest than that of group A, B, C and D (<0.05).
Decompression and Governor Vessel electro-acupuncture on acute severe upper cervical spinal cord compression injury had a better effect compare with decompression and methylprednisolone or simple decompression only, its mechanism may be related to lower the PAF levels and downregulating Caspase-9 protein expression in spinal injury tissue.
探讨减压（DE）联合督脉（GV）电针（EA）对急性重度颈髓上损伤大鼠的影响及潜在机制。
将30只SPF级大鼠随机分为5组（对照组A、B和实验组C、D、E），每组6只。通过将球囊导管置入寰枕间隙制作急性重度颈髓上损伤模型。A组为空白组，B组仅将球囊导管置入寰枕间隙但不施加压力，C、D、E组持续压迫48小时。C组接受电针干预，选取百会和大椎穴，采用连续波，频率为2Hz，治疗时间为15分钟，连续治疗14天；D组接受甲泼尼龙干预，经尾静脉注射；E组不再接受任何干预。治疗14天后获取所有大鼠的动脉血和损伤脊髓组织，采用BBB评分评估各组后肢运动功能变化，采用ELISA法检测损伤脊髓组织和血清中血小板活化因子（PAF）的含量；采用Western blot法检测治疗14天后各组Caspase-9的表达。
在6个时间点，即对照组压迫后1小时、48小时，实验组治疗后24小时、3天、7天、14天，BBB评分均为（21.000±0.000）分；实验组（C、D、E组）评分始终低于对照组（A、B组）；与E组相比，C组和D组明显更高（<0.05）；C组和D组之间无显著差异（>0.05）。ELISA法检测PAF的结果：血清PAF浓度，A、B、D、E组之间无统计学差异（>0.05），C组低于其他组（<0.05）；组织PAF浓度，A组和B组之间无显著差异（>0.05），D组明显高于A、B、C组（<0.05），E组高于其他组（<0.05）。Western blot检测显示，A组和B组Caspase-9蛋白表达相似（>0.05），C组高于A组和B组（<0.05），D组高于A、B、C组（<0.05），E组高于A、B、C、D组（<0.05）。
与减压联合甲泼尼龙或单纯减压相比，减压联合督脉电针对急性重度颈髓上损伤有更好的效果，其机制可能与降低脊髓损伤组织中PAF水平及下调Caspase-9蛋白表达有关。
