Matsuki Y, Ito T, Fukuhara K, Abe M, Othaki T, Nambara T
Arch Toxicol. 1987 Feb;59(5):374-8. doi: 10.1007/BF00295093.
A sensitive gas chromatographic-mass spectrometric method for the determination of 5-chloro-7-iodo-8-hydroxyquinoline (chinoform, clioquinol) in biological fluids and nervous tissues is described. Chinoform was converted into the pentafluorobenzyl ether, which was separated on a 10% Dexsil 300GC column and determined by the use of chinoform-d4 as an internal standard. The clean-up of chionoform in plasma and urine was efficiently achieved by extracting with benzene, while the drug in the tissue was pretreated successively by extraction with 12.5% v/v pyridine-benzene, separation on a Clin-Elut cartridge and adsorption on alumina. The quantitation limit of chinoform was 100 pg, and the recovery rates of chinoform added to plasma and tissue were 98% and 92%, respectively. The chinoform levels in biological fluids and tissues in dogs after prolonged administration of the drug at a dose of 400 mg/kg/day were measured by the proposed method. The plasma level and tissue distribution of chinoform are also discussed.
描述了一种灵敏的气相色谱 - 质谱法，用于测定生物体液和神经组织中的5 - 氯 - 7 - 碘 - 8 - 羟基喹啉（氯碘喹啉）。氯碘喹啉被转化为五氟苄基醚，在10% Dexsil 300GC柱上进行分离，并以氯碘喹啉 - d4作为内标进行测定。通过用苯萃取可有效实现血浆和尿液中氯碘喹啉的净化，而组织中的药物则依次用12.5% v/v吡啶 - 苯萃取、在Clin - Elut柱上分离并吸附在氧化铝上进行预处理。氯碘喹啉的定量限为100 pg，添加到血浆和组织中的氯碘喹啉回收率分别为98%和92%。采用所提出的方法测定了以400 mg/kg/天的剂量长期给药后犬生物体液和组织中的氯碘喹啉水平。还讨论了氯碘喹啉的血浆水平和组织分布。
