兔骨骼肌肌浆网中钙离子依赖性ATP酶的单（ADP - 核糖基）化作用及聚L - 赖氨酸的影响

Mono(ADP-ribosyl)ation of Ca2+-dependent ATPase in rabbit skeletal muscle sarcoplasmic reticulum and the effect of poly L-lysine.

作者信息

Hara N, Mishima K, Tsuchiya M, Tanigawa Y, Shimoyama M

出版信息

Biochem Biophys Res Commun. 1987 Apr 29;144(2):856-62. doi: 10.1016/s0006-291x(87)80043-8.

Abstract

We investigated the endogenous mono(ADP-ribosyl)ation of the sarcoplasmic reticulum from rabbit skeletal muscle. The autoradiogram obtained after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the [adenylate-32P]NAD-treated sarcoplasmic reticulum vesicles revealed a major band corresponding to the MW 105 K Ca2+-dependent ATPase and other bands corresponding to proteins of MW 153, 60 and 38 K and those of 125 to 135 K range. The addition of poly L-lysine during the incubation led to an enhancement of the modification. Poly L-lysine is proving to be a pertinent tool for identifying acceptor proteins.

摘要

我们研究了兔骨骼肌肌浆网的内源性单（ADP-核糖基）化作用。用[腺苷酸-32P]NAD处理肌浆网囊泡，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后得到的放射自显影片显示，有一条主要条带对应于分子量为105K的钙依赖性ATP酶，还有其他条带对应于分子量为153K、60K和38K的蛋白质以及分子量在125至135K范围内的蛋白质。孵育期间添加聚L-赖氨酸会导致修饰作用增强。聚L-赖氨酸被证明是鉴定受体蛋白的一种相关工具。