a Micropathology Ltd., University of Warwick Science Park, Coventry, United Kingdom.
b School of Life Sciences, University of Warwick, Coventry, United Kingdom.
Genome. 2018 May;61(5):379-385. doi: 10.1139/gen-2017-0195. Epub 2018 Mar 13.
Comparison of the genome of the Gram negative human pathogen Haemophilus quentini MP1 with other species of Haemophilus revealed that, although it is more closely related to Haemophilus haemolyticus than Haemophilus influenzae, the pathogen is in fact genetically distinct, a finding confirmed by phylogenetic analysis using the H. influenzae multilocus sequence typing genes. Further comparison with two other H. quentini strains recently identified in Canada revealed that these three genomes are more closely related than any other species of Haemophilus; however, there is still some sequence variation. There was no evidence of acquired antimicrobial resistance within the H. quentini MP1 genome nor any mutations within the DNA gyrase or topoisomerase IV genes known to confer resistance to fluoroquinolones, which has been previously identified in other H. quentini isolates. We hope by presenting the annotation and genetic comparison of the H. quentini MP1 genome it will aid the future molecular detection of this potentially emerging pathogen via the identification of unique genes that differentiate it from other species of Haemophilus.
将革兰氏阴性人类病原体亨氏流感嗜血杆菌 MP1 的基因组与其他种属的流感嗜血杆菌进行比较后发现，尽管它与溶血性流感嗜血杆菌的亲缘关系比流感嗜血杆菌更为密切，但实际上该病原体在基因上是独特的，这一发现通过使用流感嗜血杆菌多位点序列分型基因进行系统发育分析得到了证实。进一步与最近在加拿大发现的另外两种亨氏流感嗜血杆菌菌株进行比较后发现，这三个基因组比任何其他种属的流感嗜血杆菌都更为密切相关；然而，仍然存在一些序列差异。在亨氏流感嗜血杆菌 MP1 基因组中没有发现获得性抗微生物耐药性的证据，也没有发现已知可赋予氟喹诺酮类药物耐药性的 DNA 回旋酶或拓扑异构酶 IV 基因的突变，而在其他亨氏流感嗜血杆菌分离株中已经发现了这些突变。我们希望通过呈现亨氏流感嗜血杆菌 MP1 基因组的注释和遗传比较，将有助于通过鉴定出将其与其他种属的流感嗜血杆菌区分开来的独特基因，从而促进未来对这种潜在新兴病原体的分子检测。
