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体外共孵育 PCB-DL（PCB118）和大体积 PCB（PCB153）与 BaP 对脂肪生成和促炎状态相关基因表达的影响。

In Vitro Cocktail Effects of PCB-DL (PCB118) and Bulky PCB (PCB153) with BaP on Adipogenesis and on Expression of Genes Involved in the Establishment of a Pro-Inflammatory State.

机构信息

Aix Marseille Univ, Univ Avignon, CNRS, IRD, IMBE, Faculté de Pharmacie 27 Bd Jean Moulin, 13385 Marseille CEDEX 5, France.

出版信息

Int J Mol Sci. 2018 Mar 13;19(3):841. doi: 10.3390/ijms19030841.

DOI:10.3390/ijms19030841
PMID:29534036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5877702/
Abstract

(1) Objective: Highlight the in vitro effects of 3T3-L1 cell exposure to polychlorinated biphenyls (PCB118 and 153) or benzo(a)pyrene (BaP) alone or as a cocktail on adipogenesis (ADG) by focusing on changes in lipid metabolism and inflammatory-related genes expression (INFG) and ADG-related genes expression (ADGG); (2) Results: Treatment from the early stage of cell differentiation by BaP alone or in combination with PCBs decreased the expression of some of the ADGG (, , , , and ). BaP enhanced the INFG, especially and . Co-exposure to BaP and PCB153 showed a synergistic effect on and expression. Treatment with BaP and PCBs during only the maturation period up-regulated the INFG (, , ). PCB118 alone also enhanced , , and expression. The change in MCP-1 protein expression was in agreement with that of the gene. Finally, the BaP-induced up-regulation of the xenobiotic responsive element (XRE)-controlled luciferase activity was impaired by PCB153 but not by PCB118; (3) Conclusion: BaP and PCBs down-regulate a part of ADGG and enhance INFG. The direct regulatory effect of PCBs on both ADGG and INFG is usually rather lower than that of BaP and synergistic or antagonistic cocktail effects are clearly observed.

摘要

（1）目的：通过聚焦脂质代谢和炎症相关基因表达（INFG）和脂肪生成（ADG）相关基因表达（ADGG）的变化，强调 3T3-L1 细胞暴露于单独的多氯联苯（PCB118 和 153）或苯并（a）芘（BaP）或鸡尾酒对脂肪生成（ADG）的体外影响；（2）结果：BaP 单独或与 PCBs 联合在细胞分化的早期阶段处理会降低某些 ADGG（、、、和）的表达。BaP 增强了 INFG，特别是和。BaP 和 PCB153 的共暴露显示出对和表达的协同作用。仅在成熟阶段用 BaP 和 PCBs 处理会上调 INFG（、、）。PCB118 单独也增强了、和的表达。MCP-1 蛋白表达的变化与基因表达的变化一致。最后，PCB153 但不是 PCB118 削弱了 BaP 诱导的外源性反应元件（XRE）控制的荧光素酶活性的上调；（3）结论：BaP 和 PCBs 下调一部分 ADGG 并增强 INFG。PCBs 对 ADGG 和 INFG 的直接调节作用通常低于 BaP，并且观察到明显的协同或拮抗鸡尾酒作用。

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