弥漫性大B细胞淋巴瘤中C-MYC蛋白表达与基因异常的相关性
[Correlation between C-MYC protein expression and genetic abnormalities in diffuse large B-cell lymphoma].
作者信息
Yu H, Wang H, Zhang N, Gao S M, Zhang Y X
机构信息
Department of Pathology, Weifang Medical University, Shandong Province, Weifang 261053, China.
出版信息
Zhonghua Bing Li Xue Za Zhi. 2018 Mar 8;47(3):172-175. doi: 10.3760/cma.j.issn.0529-5807.2018.03.005.
To study the correlation between expression of oncogene C-MYC protein and gene abnormality in diffuse large B-cell lymphoma (DLBCL). The expression of C-MYC protein and gene abnormality were detected by immunohistochemistry and fluorescence in situ hybridization (FISH), respectively, in 42 cases of paraffin-embedded DLBCL. All cases were collected at Department of Pathology, Weifang People's Hospital during January 2015 to October 2016. The positive rate of C-MYC protein expression was 47.6% (20/42) and the rate of abnormal C-MYC gene by FISH was 26.2%(11/42), including translocation (23.8%, 10/42) and gene amplification (2.4%, 1/42). There was a close relationship between the protein expression and gene translocation (χ(2)=11.813; =0.001) and gene translocation occurred primarily in GCB (χ(2)=4.029; =0.045). The high expression (≥40%) of C-MYC protein is associated with its gene translocation, suggesting that C-MYC protein detection can be used as a surrogate marker for C-MYC gene translocation in DLBCL.
研究弥漫性大B细胞淋巴瘤（DLBCL）中癌基因C-MYC蛋白表达与基因异常之间的相关性。采用免疫组织化学和荧光原位杂交（FISH）技术分别检测42例石蜡包埋的DLBCL中C-MYC蛋白的表达和基因异常情况。所有病例均收集于潍坊市人民医院病理科2015年1月至2016年10月期间。C-MYC蛋白表达阳性率为47.6%（20/42），FISH检测C-MYC基因异常率为26.2%（11/42），包括易位（23.8%，10/42）和基因扩增（2.4%，1/42）。蛋白表达与基因易位之间存在密切关系（χ²=11.813；P=0.001），且基因易位主要发生在生发中心B细胞（GCB）（χ²=4.029；P=0.045）。C-MYC蛋白高表达（≥40%）与其基因易位相关，提示C-MYC蛋白检测可作为DLBCL中C-MYC基因易位的替代标志物。
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