Department of Food and Nutrition, Seoul National University, Seoul 08826, Korea.
Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
Nutrients. 2018 Mar 10;10(3):332. doi: 10.3390/nu10030332.
Offspring of female rats fed either a casein (CAS) diet or a low-isoflavone soy protein isolate (SPI) diet were compared in an animal model of chronic ethanol consumption to investigate whether maternal diet regulates the adaptive responses of offspring to postnatal ethanol exposure and potentially affects the development of liver disease in later life. Female rats were fed either a CAS or an SPI diet before mating, and during pregnancy and lactation. Male offspring from the same litter were pair-fed either a control or ethanol diet for six weeks (CAS/CON, CAS/EtOH, SPI/CON, and SPI/EtOH groups). Serum aminotransferase activities and hepatic inflammatory indicators were higher in the SPI/EtOH group than in the CAS/EtOH group. Ethanol consumption increased serum homocysteine levels, hepatic -adenosylmethionine:-adenosylhomocysteine ratio, and hepatic endoplasmic reticulum stress only in offspring of SPI-fed female rats. Total and high-density lipoprotein (HDL) cholesterol levels and mRNA levels of hepatic genes involved in HDL cholesterol assembly were reduced in the SPI group in response to ethanol consumption. In conclusion, offspring of SPI-fed female rats were more susceptible to the later development of alcoholic liver disease than offspring of CAS-fed female rats. Furthermore, maternal SPI consumption altered one-carbon metabolism and cholesterol metabolism of offspring fed an ethanol diet.
在慢性乙醇摄入动物模型中，比较了食用酪蛋白（CAS）饮食或低异黄酮大豆蛋白分离物（SPI）饮食的雌性大鼠的后代，以研究母体饮食是否调节后代对产后乙醇暴露的适应性反应，并可能影响其在以后生活中肝脏疾病的发展。雌性大鼠在交配前、怀孕期间和哺乳期均食用 CAS 或 SPI 饮食。来自同一窝的雄性后代被成对喂食对照或乙醇饮食 6 周（CAS/CON、CAS/EtOH、SPI/CON 和 SPI/EtOH 组）。与 CAS/EtOH 组相比，SPI/EtOH 组的血清转氨酶活性和肝炎症指标更高。乙醇摄入仅增加了 SPI 喂养雌性大鼠后代的血清同型半胱氨酸水平、肝-腺苷甲硫氨酸：-腺苷同型半胱氨酸比和肝内质网应激。SPI 组的总胆固醇和高密度脂蛋白（HDL）胆固醇水平以及参与 HDL 胆固醇组装的肝基因的 mRNA 水平因乙醇摄入而降低。总之，与 CAS 喂养的雌性大鼠的后代相比，SPI 喂养的雌性大鼠的后代更容易发生酒精性肝病。此外，母体 SPI 消耗改变了喂食乙醇饮食的后代的一碳代谢和胆固醇代谢。
