Yoon Mi, Won Sae Bom, Kwon Young Hye
Department of Food and Nutrition, Seoul National University, Seoul 08826, Republic of Korea.
Department of Food and Nutrition, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Human Ecology, Seoul National University, Seoul 08826, Republic of Korea.
Life Sci. 2017 Apr 1;174:1-7. doi: 10.1016/j.lfs.2017.02.009. Epub 2017 Feb 20.
Substantial studies have reported that maternal protein restriction may induce later development of cardiovascular disease in offspring by impairing antioxidant system and lipid metabolism. Because a unique amino acid composition of soy protein isolate has been shown to provide health benefits, including hypolipidemic effects, we investigated effects of maternal low-protein diet composed of low-isoflavone soy protein isolate (SPI) on oxidative stress and lipid metabolism in offspring.
Sprague-Dawley dams were fed 20% or 10% SPI diet throughout pregnancy and lactation. On postnatal day 21, male offspring and their dams were studied.
Maternal consumption of low-protein diet composed of SPI did not induce hepatic oxidative stress in offspring. Although serum triacylglycerol and cholesterol levels in dams were not different between groups, serum triacylglycerol levels were lower in offspring of dams fed a 10% SPI diet (10% SPI group) compared to offspring of dams fed a 20% SPI diet (20% SPI group). Maternal protein restriction also reduced serum HDL/total cholesterol levels. The mRNA levels of apolipoprotein A1, which is required for HDL formation, were lower in 10% SPI group compared to 20% SPI group and were positively correlated with serum HDL-cholesterol levels.
Although maternal consumption of low-protein diet containing SPI did not induce oxidative stress and hypertriglyceridemia, the present study indicates that it may disturb cholesterol metabolism of rat offspring on postnatal day 21. Further studies are warranted to investigate the effect of maternal diet composed of soy protein isolate on later development of cardiovascular disease in offspring.
大量研究报告称，母体蛋白质限制可能通过损害抗氧化系统和脂质代谢，诱导后代后期发生心血管疾病。由于大豆分离蛋白独特的氨基酸组成已被证明具有健康益处，包括降血脂作用，我们研究了由低异黄酮大豆分离蛋白（SPI）组成的母体低蛋白饮食对后代氧化应激和脂质代谢的影响。
在整个怀孕和哺乳期间，给斯普拉格-道利母鼠喂食20%或10%的SPI饮食。在出生后第21天，对雄性后代及其母鼠进行研究。
母体食用由SPI组成的低蛋白饮食并未在后代中诱导肝脏氧化应激。尽管两组母鼠的血清三酰甘油和胆固醇水平没有差异，但与喂食20%SPI饮食的母鼠（20%SPI组）的后代相比，喂食10%SPI饮食的母鼠（10%SPI组）的后代血清三酰甘油水平较低。母体蛋白质限制也降低了血清高密度脂蛋白/总胆固醇水平。与20%SPI组相比，10%SPI组中高密度脂蛋白形成所需的载脂蛋白A1的mRNA水平较低，且与血清高密度脂蛋白胆固醇水平呈正相关。
尽管母体食用含SPI的低蛋白饮食未诱导氧化应激和高甘油三酯血症，但本研究表明，它可能会干扰出生后第21天大鼠后代的胆固醇代谢。有必要进一步研究由大豆分离蛋白组成的母体饮食对后代心血管疾病后期发展的影响。
