Université de Lyon, ENS de Lyon, CNRS, LGL-TPE, 69007 Lyon, France.
UMR 5534, Institut de Génomique Fonctionelle de Lyon (IGFL), CNRS, Université Claude Bernard (Lyon 1), Ecole normale supérieure de Lyon, France.
Metallomics. 2018 Mar 1;10(3):496-503. doi: 10.1039/c7mt00318h. Epub 2018 Mar 14.
Ageing is accompanied by important chemical deregulations resulting in bodily metal imbalances. The way and extent to which these deregulations are associated with ageing processes are however poorly understood and their use as potential biomarkers of ageing has not been investigated. In this study, we report whole-body elementary concentrations and copper and zinc isotopic compositions of Caenorhabditis elegans in ageing wild type (i.e.'normal'-lived) and mutant (i.e. short and long-lived) strains. We show that the strains are characterized by different levels of mutation-related variations such as in phosphorus and magnesium as well as in zinc isotopic composition. During ageing, strains are affected by elemental age-related variations, such as an increase in calcium and iron concentrations and a decrease in the copper isotopic composition and concentration for long-lived mutants. The deregulated metabolism of copper seems to be connected to ageing probably in association with the production of reactive oxygen species. We emphasize that the copper stable isotope composition could serve as a biomarker of normal or accelerated ageing in the future.
衰老是伴随着重要的化学失调，导致身体金属失衡。然而，这些失调与衰老过程的关系及其作为衰老潜在生物标志物的用途还知之甚少。在这项研究中，我们报告了秀丽隐杆线虫在衰老野生型（即“正常”寿命）和突变型（即短寿命和长寿命）菌株中的全身元素浓度以及铜和锌的同位素组成。我们表明，这些菌株具有不同水平的与突变相关的变化，如磷和镁以及锌的同位素组成。在衰老过程中，菌株受到元素年龄相关变化的影响，例如长寿命突变体的钙和铁浓度增加，以及铜同位素组成和浓度降低。铜代谢的失调似乎与衰老有关，可能与活性氧的产生有关。我们强调，铜的稳定同位素组成将来可能成为正常或加速衰老的生物标志物。
