转录组测序：RNA测序

Transcriptome Sequencing: RNA-Seq.

作者信息

Zhang Hong, He Lin, Cai Lei

机构信息

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center for Genetics and Development, Shanghai Jiaotong University, Shanghai, 200240, China.

出版信息

Methods Mol Biol. 2018;1754:15-27. doi: 10.1007/978-1-4939-7717-8_2.

DOI:10.1007/978-1-4939-7717-8_2

PMID:29536435

Abstract

RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of different platforms are not exactly the same; each platform has its own kit to meet the special requirements of the instrument design.

摘要

RNA测序（RNA-seq）不仅可用于识别常见或罕见转录本的表达，还可用于识别其他异常事件，如可变剪接、新转录本和融合基因。原则上，几乎所有的新一代测序（NGS）平台都可以进行RNA测序，但不同平台的文库并不完全相同；每个平台都有自己的试剂盒以满足仪器设计的特殊要求。