Zhang Zhenyu, Ma Xiaoming, La Yongfu, Guo Xian, Chu Min, Bao Pengjia, Yan Ping, Wu Xiaoyun, Liang Chunnian
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Key Laboratory for Yak Genetics, Breeding, and Reproduction Engineering of Gansu Province, Gansu Provincial Key Laboratory of Yak Breeding Engineering, Lanzhou Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou 730070, China.
Int J Mol Sci. 2024 Dec 22;25(24):13706. doi: 10.3390/ijms252413706.
Single-cell sequencing technology provides apparent advantages in cell population heterogeneity, allowing individuals to better comprehend tissues and organs. Sequencing technology is currently moving beyond the standard transcriptome to the single-cell level, which is likely to bring new insights into the function of breast cells. In this study, we examine the primary cell types involved in breast development, as well as achievements in the study of scRNA-seq in the microenvironment, stressing the finding of novel cell subsets using single-cell approaches and analyzing the problems and solutions to scRNA-seq. Furthermore, we are excited about the field's promising future.
单细胞测序技术在细胞群体异质性方面具有明显优势，使人们能够更好地理解组织和器官。目前，测序技术正从标准转录组迈向单细胞水平，这可能会为乳腺细胞的功能带来新的见解。在本研究中，我们考察了乳腺发育中涉及的主要细胞类型，以及单细胞RNA测序在微环境研究中的成果，着重介绍了使用单细胞方法发现的新型细胞亚群，并分析了单细胞RNA测序存在的问题及解决方案。此外，我们对该领域充满希望的未来感到兴奋。
