Fan Beiyuan, Wang Junbo, Xu Ying, Chen Jian
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Methods Mol Biol. 2018;1754:293-309. doi: 10.1007/978-1-4939-7717-8_17.
Quantification of single-cell proteomics provides key insights in the field of cellular heterogeneity. This chapter discusses the emerging techniques that are being used to measure the protein copy numbers at the single-cell level, which includes flow cytometry, mass cytometry, droplet cytometry, microengraving, and single-cell barcoding microchip. The advantages and limitations of each technique are compared, and future research opportunities are highlighted.
单细胞蛋白质组学的定量分析为细胞异质性领域提供了关键见解。本章讨论了用于在单细胞水平测量蛋白质拷贝数的新兴技术，其中包括流式细胞术、质谱流式细胞术、液滴流式细胞术、微刻技术和单细胞条形码微芯片。比较了每种技术的优缺点，并突出了未来的研究机会。
