State Key Laboratory of Separation Membranes and Membrane Processes, School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin, 300387, China.
School of Material Science and Engineering, Tianjin University of Technology, Tianjin, 300384, China.
J Biomed Mater Res B Appl Biomater. 2018 Nov;106(8):2869-2877. doi: 10.1002/jbm.b.34106. Epub 2018 Mar 14.
Membrane surface design is significant for the development and application of synthetic polymer hemodialysis membranes. In this study, the influence of zwitterionic cysteine on poly(vinylidene fluoride) (PVDF) hollow fiber membrane was investigated. The polydopamine layer was formed through dopamine self-polymerization on PVDF membrane surface, and then cysteine was covalent grafted onto the layer to improve the anti-biofouling property and hemocompatibility. The elementary composition of membrane surfaces was characterized by X-ray photoelectron spectroscopy. The influence of polydopamine and cysteine on modified membrane surface morphologies was studied by field emission scanning electron microscopy. The modified PVDF membranes were confirmed to have excellent hydrophilicity, stable mechanical properties and good hemocompatibility (dynamic and static anti-protein adsorption, hemolysis ratio, plasma coagulation). And these properties were increased with the incorporation of polydopamine and cysteine. The optimized modified membranes exhibited high pure water flux (∼ 195.5 L/m h at 0.1 MPa) and selectivity (clearance ratio of urea and lysozyme was 75.1 and 55.4%, and rejection rate of bovine serum albumin was 98.8%). This work provides a surface modification method of PVDF hollow fiber membranes and suggests a potential application of PVDF membranes in hemodialysis field. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2869-2877, 2018.
膜表面设计对于合成聚合物血液透析膜的开发和应用具有重要意义。本研究考察了两性离子半胱氨酸对聚偏二氟乙烯（PVDF）中空纤维膜的影响。通过多巴胺在 PVDF 膜表面的自聚合形成聚多巴胺层，然后将半胱氨酸共价接枝到该层上，以提高抗生物污染性能和血液相容性。采用 X 射线光电子能谱对膜表面的基本组成进行了表征。用场发射扫描电子显微镜研究了聚多巴胺和半胱氨酸对改性膜表面形貌的影响。改性后的 PVDF 膜具有优异的亲水性、稳定的机械性能和良好的血液相容性（动态和静态抗蛋白吸附、溶血率、血浆凝固），并且这些性能随着聚多巴胺和半胱氨酸的加入而增加。优化后的改性膜表现出较高的纯水通量（在 0.1 MPa 下约为 195.5 L/m h）和选择性（尿素和溶菌酶的清除率分别为 75.1%和 55.4%，牛血清白蛋白的截留率为 98.8%）。这项工作为 PVDF 中空纤维膜的表面改性方法提供了一种思路，并为 PVDF 膜在血液透析领域的潜在应用提供了参考。© 2018 年威利父子公司。J 生物医学材料研究部分 B：应用生物材料，106B：2869-2877，2018 年。
