Kabatas S, Demir C S, Civelek E, Yilmaz I, Kircelli A, Yilmaz C, Akyuva Y, Karaoz E
Bratisl Lek Listy. 2018;119(3):143-151. doi: 10.4149/BLL_2018_028.
This study aimed to analyze the effect of human Dental Pulp-Neural Crest Stem Cells (hDP-NCSCs) delivery on lesion site after spinal cord injury (SCI), and to observe the functional recovery after transplantation.
Neural Crest Stem Cells (NCSCs) were isolated from human Dental Pulp (hDP). The experimental rat population was divided into four groups (n = 6/24). Their behavioral motility was scored regularly. After 4-weeks, rats were sacrificed, and their spinal cords were examined for Green Fluorescent Protein (GFP) labeled hDP-NCSCs by immunofluorescence (IF) staining.
In early post-injury (p.i) period, the ultrastructure of spinal cord tissue was preserved in Group 4. The majority of cells forming the ependymal region around the central canal were found to be hDP-NCSCs. While the grey-and-white-matter around the ependymal region was composed of e.g. GFP cells, with astrocytic-like appearance. The scores showed significant motor recovery in hind limb functions in Group 4. However, no obvious change was observed in other groups.
Cells e.g., mesenchymal (Vimentin+) which express GFP+ cells in the gray-and-white-matter around the ependymal region could indicate the potential to self-renewal and plasticity. Thus, transplantation of hDP-NCSCs might be an effective strategy to improve functional recovery following spinal cord trauma (Fig. 10, Ref. 32).
本研究旨在分析人牙髓神经嵴干细胞（hDP-NCSCs）移植对脊髓损伤（SCI）后损伤部位的影响，并观察移植后的功能恢复情况。
从人牙髓（hDP）中分离神经嵴干细胞（NCSCs）。将实验大鼠分为四组（n = 6/24）。定期对其行为运动能力进行评分。4周后，处死大鼠，通过免疫荧光（IF）染色检查其脊髓中绿色荧光蛋白（GFP）标记的hDP-NCSCs。
在损伤后早期（p.i），第4组脊髓组织的超微结构得以保留。发现中央管周围形成室管膜区域的大多数细胞为hDP-NCSCs。而室管膜区域周围的灰质和白质由例如具有星形胶质细胞样外观的GFP细胞组成。评分显示第4组后肢功能有明显的运动恢复。然而，其他组未观察到明显变化。
例如在室管膜区域周围的灰质和白质中表达GFP+细胞的间充质细胞（波形蛋白+）可能表明其具有自我更新和可塑性的潜力。因此，hDP-NCSCs移植可能是改善脊髓创伤后功能恢复的有效策略（图10，参考文献32）。
